Molecular characterization and genetic diversity of Wolbachia endosymbionts in bed bugs (Hemiptera; Cimicidae) collected in Paris.

Autor: Chebbah D; Parasitology-Mycology Department, Avicenne Hospital, AP-HP, Bobigny, France.; Service Parisien de Santé Environnementale (SPSE), Sous-Direction de la Santé Environnementale et de la Prévention (SDSEP), Direction de la Santé Publique (DSP)-Mairie de Paris, Paris, France., Hamarsheh O; Department of Biological Sciences, Al-Quds University, Jerusalem, Palestine., Sereno D; Institut de Recherche pour le Développement, MIVEGEC, Montpellier, France.; Institut de Recherche pour le Développement, InterTryp, Montpellier, France., Elissa N; Service Parisien de Santé Environnementale (SPSE), Sous-Direction de la Santé Environnementale et de la Prévention (SDSEP), Direction de la Santé Publique (DSP)-Mairie de Paris, Paris, France., Brun S; Parasitology-Mycology Department, Avicenne Hospital, AP-HP, Bobigny, France., Jan J; Agence Régionale de Santé (ARS) Île-de-France, Paris, France., Izri A; Parasitology-Mycology Department, Avicenne Hospital, AP-HP, Bobigny, France.; Unité des Virus Émergents (UVE: Aix-Marseille Univ-IRD 190-Inserm 1207-IHU Méditerranée Infection), Marseille, France., Akhoundi M; Parasitology-Mycology Department, Avicenne Hospital, AP-HP, Bobigny, France.; Unité des Virus Émergents (UVE: Aix-Marseille Univ-IRD 190-Inserm 1207-IHU Méditerranée Infection), Marseille, France.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2023 Sep 28; Vol. 18 (9), pp. e0292229. Date of Electronic Publication: 2023 Sep 28 (Print Publication: 2023).
DOI: 10.1371/journal.pone.0292229
Abstrakt: Purpose: This study aimed to investigate the genetic diversity of Wolbachia in field-caught bed bug species in Paris areas.
Methods: The bed bug specimens were captured from various infested localities in Paris and surrounding cities. They belonged to diverse life stages, including egg, nymph, and adult. They were then identified using morphological and molecular approaches. Furthermore, Wolbachia was detected, and its genetic diversity was investigated by conventional PCR of 16S-rRNA and Wolbachia surface protein (wsp) genes.
Results: A total of 256 bed bug specimens belonging to various life stages [adult (183 specimens), nymph (48), and egg (25)] were captured from seven private apartments, five social apartments, three houses, two immigrant residences, and one retirement home situated in 10 districts of Paris and 8 surrounding cities. They were identified as Cimex lectularius (237 specimens) and C. hemipterus (19) using morphological and molecular approaches. The presence and diversity of Wolbachia were ascertained by targeting 16S-rRNA and wsp genes. Based on molecular analysis, 182 and 148 out of 256 processed specimens were positive by amplifying 16S-rRNA and wsp fragments, respectively. The inferred phylogenetic analysis with 16S-rRNA and wsp sequences displayed monophyletic Wolbachia strains clustering each one in three populations. The median-joining network, including the Wolbachia 16S-rRNA and wsp sequences of C. lectularius and C. hemipterous specimens, indicated a significant genetic differentiation among these populations in Paris areas which was consent with Neighbor-Joining analyses. A phylogenetic analysis of our heterogenic Wolbachia sequences with those reported from other arthropod species confirmed their belonging to supergroup F. Moreover, no difference between Wolbachia sequences from eggs, nymphs, and adults belonging to the same clade and between Wolbachia sequences of C. lectularius and C. hemipterus were observed after sequence alignment. Furthermore, no significant correlation was found between multiple geographical locations (or accomodation type) where bed bugs were collected and the genetic diversity of Wolbachia.
Conclusions: We highlight a significant heterogeneity within Wolbachia symbionts detected in C. lectularius and C. hemipterus. No correlation between Wolbachia species and bed bug species (C. lectularius versus C. hemipterus), physiological stages (egg, nymph, and adult), and sampling location was recorded in this study.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Chebbah et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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