Development of a Method for Isolation of Mature Cardiomyocytes from Human Heart Biopsy Specimens.

Autor: Kovalenko SG; Research Laboratory of Molecular and Cellular Diagnostics, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia.; Laboratory of Experimental and Cellular Medicine, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Moscow region, Russia., Frolova SR; Research Laboratory of Molecular and Cellular Diagnostics, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia.; Laboratory of Experimental and Cellular Medicine, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Moscow region, Russia., Kramkova VK; Laboratory of Experimental and Cellular Medicine, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Moscow region, Russia., Berezovskii AK; Laboratory of Experimental and Cellular Medicine, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Moscow region, Russia., Popov MA; Department of Heart and Vessels Surgery, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia., Shumakov DV; Department of Heart and Vessels Surgery, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia., Zybin DI; Department of Heart and Vessels Surgery, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia., Agafonov EG; Department of Heart and Vessels Surgery, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia., Dontsov VV; Department of Heart and Vessels Surgery, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia., Agladze KI; Research Laboratory of Molecular and Cellular Diagnostics, M. F. Vladimirsky Moscow Region Research Clinical Institute, Moscow, Russia. agladze@yahoo.com.; Laboratory of Experimental and Cellular Medicine, Moscow Institute of Physics and Technology (National Research University), Dolgoprudny, Moscow region, Russia. agladze@yahoo.com.
Jazyk: angličtina
Zdroj: Bulletin of experimental biology and medicine [Bull Exp Biol Med] 2023 Aug; Vol. 175 (4), pp. 585-591. Date of Electronic Publication: 2023 Sep 28.
DOI: 10.1007/s10517-023-05907-x
Abstrakt: To increase the yield of living cells and their survival, studies were carried out to optimize the method for isolating cardiomyocytes from biopsy specimens excised from the right atrial appendages. It was found that creatine, blebbistatin, and taurine are necessary components of the buffer solution during cardiomyocyte isolation, and that composition of the solutions is a more important factor than their oxygenation.
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Databáze: MEDLINE
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