Mincing bovine articular cartilage with commercially available shavers reduces the viability of chondrocytes compared to scalpel mincing.

Autor: Moser LB; Center for Regenerative Medicine, Department for Health Sciences, Medicine and Research, University for Continuing Education Krems, Dr.-Karl-Dorrek-Straße 30, 3500, Krems, Austria. lukas.moser@donau-uni.ac.at.; Department of Orthopaedics and Traumatology, University Hospital Krems, 3500, Krems, Austria. lukas.moser@donau-uni.ac.at., Bauer C; Center for Regenerative Medicine, Department for Health Sciences, Medicine and Research, University for Continuing Education Krems, Dr.-Karl-Dorrek-Straße 30, 3500, Krems, Austria., Otahal A; Center for Regenerative Medicine, Department for Health Sciences, Medicine and Research, University for Continuing Education Krems, Dr.-Karl-Dorrek-Straße 30, 3500, Krems, Austria., Kern D; Center for Regenerative Medicine, Department for Health Sciences, Medicine and Research, University for Continuing Education Krems, Dr.-Karl-Dorrek-Straße 30, 3500, Krems, Austria., Dammerer D; Department of Orthopaedics and Traumatology, University Hospital Krems, 3500, Krems, Austria., Zantop T; Center for Regenerative Medicine, Department for Health Sciences, Medicine and Research, University for Continuing Education Krems, Dr.-Karl-Dorrek-Straße 30, 3500, Krems, Austria.; Sporthopaedicum Straubing, Straubing, Germany.; Department for Health Sciences, Medicine and Research, University for Continuing Education Krems, 3500, Krems, Austria., Nehrer S; Center for Regenerative Medicine, Department for Health Sciences, Medicine and Research, University for Continuing Education Krems, Dr.-Karl-Dorrek-Straße 30, 3500, Krems, Austria.; Department of Orthopaedics and Traumatology, University Hospital Krems, 3500, Krems, Austria.
Jazyk: angličtina
Zdroj: Journal of experimental orthopaedics [J Exp Orthop] 2023 Sep 28; Vol. 10 (1), pp. 97. Date of Electronic Publication: 2023 Sep 28.
DOI: 10.1186/s40634-023-00661-5
Abstrakt: Purpose: The study aimed to compare the effect of mincing bovine articular cartilage with different shaver blades on chondrocyte viability.
Methods: Bovine articular cartilage was harvested either with a scalpel or with three different shaver blades (2.5 mm, 3.5 mm, or 4.2 mm) from a commercially available shaver. The cartilage harvested with a scalpel was then minced into fragments smaller than 1 mm 3 with a scalpel. All four conditions were cultivated in a culture medium for seven days. After Day 1 and Day 7, the following measurements were performed: metabolic activity, RNA isolation, and gene expression of anabolic (COL2A1 and ACAN) and catabolic genes (MMP1 and MMP13), live/dead staining and visualization using confocal microscopy, and flow cytometric characterization of minced cartilage chondrocytes.
Results: Mincing the cartilage with shavers significantly reduced metabolic activity after one and seven days compared to scalpel mincing (p < 0.001). Gene expression of anabolic genes (COL2A1 and ACAN) was reduced, while catabolic genes (MMP1 and MMP13) were increased after day 7 in all shaver conditions. Confocal microscopy showed a thin line of dead cells at the lesion side with viable cells beneath for the scalpel mincing and a higher number of dead cells diffusely distributed in the shaver conditions. After seven days, there was a significant decrease in viable cells in the shaver conditions compared to scalpel mincing (p < 0.05). Flow cytometric characterization revealed fewer intact cells and proportionally more dead cells in all shaver conditions compared to the scalpel mincing.
Conclusion: Mincing bovine articular cartilage with commercially available shavers reduces the viability of chondrocytes compared to scalpel mincing immediately after harvest and after seven days in culture. This suggests that mincing cartilage with a shaver should be considered a matrix rather than a cell therapy.
Level of Evidence: Level II therapeutic study.
(© 2023. European Society of Sports Traumatology, Knee Surgery, Arthroscopy (ESSKA).)
Databáze: MEDLINE
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