| Autor: |
Ben Saad H; Laboratory of Medicinal and Environment Chemistry, Higher Institute of Biotechnology, University of Sfax, Sfax 3000, Tunisia., Frikha D; Laboratory of Marine Biodiversity and Environment, University of Sfax, Sfax 3000, Tunisia., Bouallegue A; Laboratory for the Improvement of Plants and Valorization of Agroressources, National School of Engineering of Sfax, Sfax 3038, Tunisia., Badraoui R; Laboratory of General Biology, University of Ha'il, Ha'il 45851, Saudi Arabia.; Section of Histology-Cytology, Medicine Faculty of Tunis, University of Tunis El Manar, Tunis 1007, Tunisia., Mellouli M; Anatomopathology Laboratory, University of Sfax, Habib Bourguiba Hospital, Sfax 3029, Tunisia., Kallel H; Intensive Care Unit, Cayenne General Hospital, Cayenne 97300, French Guiana.; Tropical Biome and Immunopathology, Inserm U 1019, University of Guyane, Cayenne 97346, French Guiana., Pujo JM; Emergency Department, Cayenne General Hospital, Cayenne 97300, French Guiana., Ben Amara I; Laboratory of Medicinal and Environment Chemistry, Higher Institute of Biotechnology, University of Sfax, Sfax 3000, Tunisia. |
| Abstrakt: |
Sulfated polysaccharides from seaweed are highly active natural substances with valuable applications. In the present paper, attempts have been made to discuss the physicochemical and structural features of polysaccharides isolated from red marine alga Alsidium corallinum (ACPs) and their protective effect in hepatic impairments induced by tebuconazole (TEB) in male adult rats. Structural features were determined using high-performance liquid chromatography, Fourier-transformed infrared, and solid-state 1 H and 13 C-Nuclear magnetic resonance analysis. ACPs are found to be hetero-sulfated-anionic polysaccharides that contain carbohydrates, sulfate groups, and uronic acids. In vitro biological activities suggested the effective antioxidant and antimicrobial capacities of ACPs. For antioxidant testing in vivo, the biochemical analysis and plasma profiles displayed that oral administration of ACPs could mitigate blood lipid indicators, including total cholesterol, triglyceride, low and high-density lipoprotein cholesterol, and bilirubin. Liver function indexes involving alanine aminotransferase and aspartate aminotransferase showed that ACPs possessed prominent antioxidant activities. Additionally, the intervention of ACPs potentially inhibited lipid peroxidation, protein oxidation, key enzymes of lipid metabolism (<0.001), and improved antioxidant status (<0.05). Histomorphological observation confirmed that ACPs intervention could partially repair liver injuries caused by TEB. The computational results showed that A. corallinum monosaccharides bound 1JIJ, 1HD2, and 1WL4 receptors with acceptable affinities, which, together with deep embedding and molecular interactions, support the antioxidant, antimicrobial, and hypolipidemic outlined effects in the in vitro and in vivo findings. Given their prominent antioxidant effects, ACPs are promising candidates for liver diseases and must be considered in pharmaceutical applications. |
