| Autor: |
Tosto R; Institute of Crystallography, National Research Council of Italy (CNR), P. Gaifami 18, 95126 Catania, Italy., Vecchio G; Department of Chemical Sciences, University of Catania, A. Doria 6, 95125 Catania, Italy., Bellia F; Institute of Crystallography, National Research Council of Italy (CNR), P. Gaifami 18, 95126 Catania, Italy. |
| Jazyk: |
angličtina |
| Zdroj: |
Molecules (Basel, Switzerland) [Molecules] 2023 Sep 20; Vol. 28 (18). Date of Electronic Publication: 2023 Sep 20. |
| DOI: |
10.3390/molecules28186724 |
| Abstrakt: |
Neurodegenerative diseases affect millions of people worldwide. The failure of the enzymatic degradation, the oxidative stress, the dyshomeostasis of metal ions, among many other biochemical events, might trigger the pathological route, but the onset of these pathologies is unknown. Multi-target and multifunctional molecules could address several biomolecular issues of the pathologies. The tripeptide GHK, a bioactive fragment of several proteins, and the related copper(II) complex have been largely used for many purposes, from cosmetic to therapeutic applications. GHK derivatives were synthesized to increase the peptide stability and improve the target delivery. Herein we report the synthesis of a new biotin-GHK conjugate (BioGHK) through orthogonal reactions. BioGHK is still capable of coordinating copper(II), as observed by spectroscopic and spectrometric measurements. The spectroscopic monitoring of the copper-induced ascorbate oxidation was used to measure the antioxidant activity Cu(II)-BioGHK complex, whereas antiglycant activity of the ligand towards harmful reactive species was investigated using MALDI-TOF. The affinity of BioGHK for streptavidin was evaluated using a spectrophotometric assay and compared to that of biotin. Finally, the antiaggregant activity towards amyloid-β was evaluated using a turn-on fluorescent dye. BioGHK could treat and/or prevent several adverse biochemical reactions that characterize neurodegenerative disorders, such as Alzheimer's disease. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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