| Autor: |
Barreira T; Laboratory and Facility Multi-User, Evandro Chagas National Institute of Infectious Diseases, FIOCRUZ, Rio de Janeiro 21040-360, Brazil., Corrêa-Moreira D; Laboratory of Taxonomy, Biochemistry and Bioprospecting of Fungi, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro 21040-360, Brazil., de Moraes Borba C; Laboratory of Taxonomy, Biochemistry and Bioprospecting of Fungi, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro 21040-360, Brazil., Menezes RC; Laboratory of Clinical Research in Dermatozoonoses in Domestic Animals, Evandro Chagas National Institute of Infectious Diseases, FIOCRUZ, Rio de Janeiro 21040-360, Brazil., de Moraes AML; Laboratory of Taxonomy, Biochemistry and Bioprospecting of Fungi, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro 21040-360, Brazil., Oliveira MME; Laboratory of Taxonomy, Biochemistry and Bioprospecting of Fungi, Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro 21040-360, Brazil. |
| Abstrakt: |
In an attempt to determine the mating type of different Sporothrix schenckii sensu stricto isolates that remained viable after a long period of preservation in a culture collection and to correlate them with the degree of virulence/pathogenicity, a PCR technique using primers designed for the sequences of MAT1-1-1 and MAT1-2-1 genes and a murine experimental model were used. The results showed that there was no correlation between the mating type and virulence among the isolates. Furthermore, different degrees of virulence/pathogenicity, ranging from high to low, were found among them based on different virulence parameters. It was assumed that the long period of preservation favored the changes, yielding the isolation of variants. Thus, we believe that new technologies for studies on factors can improve our knowledge of the pathogenesis of sporotrichosis. |
