| Autor: |
Ramzan F; Liggins Institute, The University of Auckland, Auckland 1023, New Zealand., Sequeira-Bisson IR; The High-Value Nutrition National Science Challenge, Auckland 1023, New Zealand.; Human Nutrition Unit, School of Biological Sciences, Faculty of Science, The University of Auckland, Auckland 1010, New Zealand.; The Riddet Institute, Massey University, Palmerston North 4410, New Zealand., Lu LW; The High-Value Nutrition National Science Challenge, Auckland 1023, New Zealand.; Human Nutrition Unit, School of Biological Sciences, Faculty of Science, The University of Auckland, Auckland 1010, New Zealand., Mitchell CJ; School of Kinesiology, The University of British Columbia, Vancouver, BC V6T 1Z1, Canada., D'Souza RF; School of Medical Sciences, The University of Auckland, Auckland 1023, New Zealand., Vickers MH; Liggins Institute, The University of Auckland, Auckland 1023, New Zealand., Poppitt SD; The High-Value Nutrition National Science Challenge, Auckland 1023, New Zealand.; Human Nutrition Unit, School of Biological Sciences, Faculty of Science, The University of Auckland, Auckland 1010, New Zealand.; The Riddet Institute, Massey University, Palmerston North 4410, New Zealand.; Department of Medicine, The University of Auckland, Auckland 1023, New Zealand., Cameron-Smith D; Liggins Institute, The University of Auckland, Auckland 1023, New Zealand.; School of Agriculture, Food and Ecosystem Sciences, The University of Melbourne, Melbourne 3010, Australia. |
| Abstrakt: |
Ectopic lipid accumulation, including intra-pancreatic fat deposition (IPFD), exacerbates type 2 diabetes risk in susceptible individuals. Dysregulated circulating microRNAs (miRNAs) have been identified as correlating with clinical measures of pancreatitis, pancreatic cancer and type 1 diabetes. The aim of the current study was therefore to examine the association between circulating abundances of candidate miRNAs, IPFD and liver fat deposition as quantified using magnetic resonance imaging (MRI) and spectroscopy (MRS). Asian Chinese ( n = 34; BMI = 26.7 ± 4.2 kg/m 2 ) and European Caucasian ( n = 34; BMI = 28.0 ± 4.5 kg/m 2 ) females from the TOFI_Asia cohort underwent MRI and MRS analysis of pancreas (MR-%IPFD) and liver fat (MR-%liver fat), respectively, to quantify ectopic lipid deposition. Plasma miRNA abundances of a subset of circulatory miRNAs associated with IPFD and liver fat deposition were quantified by qRT-PCR. miR-21-3p and miR-320a-5p correlated with MR-%IPFD, plasma insulin and HOMA2-IR, but not MR-%liver fat. MR-%IPFD remained associated with decreasing miR-21-3p abundance following multivariate regression analysis. miR-21-3p and miR-320a were demonstrated to be negatively correlated with MR-%IPFD, independent of ethnicity. For miR-21-3p, this relationship persists with the inclusion of MR-%liver fat in the model, suggesting the potential for a wider application as a specific circulatory correlate of IPFD. |
