| Autor: |
Pioltine EM; Multi-User Laboratory of Phytomedicines Pharmacology, and Biotechnology (PhitoPharmaTec), Department of Pharmacology, Institute of Biosciences, São Paulo State University (UNESP), Botucatu 18618-000, Brazil., Costa CB; Multi-User Laboratory of Phytomedicines Pharmacology, and Biotechnology (PhitoPharmaTec), Department of Pharmacology, Institute of Biosciences, São Paulo State University (UNESP), Botucatu 18618-000, Brazil.; Laboratory of Embryonic Micromanipulation, Department of Biological Sciences, School of Sciences and Languages, São Paulo State University (UNESP), Assis 19806-900, Brazil., Franchi FF; Multi-User Laboratory of Phytomedicines Pharmacology, and Biotechnology (PhitoPharmaTec), Department of Pharmacology, Institute of Biosciences, São Paulo State University (UNESP), Botucatu 18618-000, Brazil., Dos Santos PH; Multi-User Laboratory of Phytomedicines Pharmacology, and Biotechnology (PhitoPharmaTec), Department of Pharmacology, Institute of Biosciences, São Paulo State University (UNESP), Botucatu 18618-000, Brazil., Nogueira MFG; Multi-User Laboratory of Phytomedicines Pharmacology, and Biotechnology (PhitoPharmaTec), Department of Pharmacology, Institute of Biosciences, São Paulo State University (UNESP), Botucatu 18618-000, Brazil.; Laboratory of Embryonic Micromanipulation, Department of Biological Sciences, School of Sciences and Languages, São Paulo State University (UNESP), Assis 19806-900, Brazil. |
| Abstrakt: |
During embryo development, the endoplasmic reticulum (ER) acts as an important site for protein biosynthesis; however, in vitro culture (IVC) can negatively affect ER homeostasis. Therefore, the aim of our study was to evaluate the effects of the supplementation of tauroursodeoxycholic acid (TUDCA), an ER stress inhibitor, in the IVC of bovine embryos. Two experiments were carried out: Exp. 1: an evaluation of blastocyst rate, hatching kinetics, and gene expression of hatched embryos after being treated with different concentrations of TUDCA (50, 200, or 1000 μM) in the IVC; Exp. 2: an evaluation of the re-expansion, hatching, and gene expression of hatched embryos previously treated with 200 µM of TUDCA at IVC and submitted to vitrification. There was no increase in the blastocyst and hatched blastocyst rates treated with TUDCA in the IVC. However, embryos submitted to vitrification after treatment with 200 µM of TUDCA underwent an increased hatching rate post-warming together with a down-regulation in the expression of ER stress-related genes and the accumulation of lipids. In conclusion, this work showed that the addition of TUDCA during in vitro culture can improve the cryotolerance of the bovine blastocyst through the putative modulation of ER and oxidative stress. |
