| Autor: |
Bukomarhe CB; Department of Horticulture and Food Security, Jomo Kenyatta University of Agriculture and Technology (JKUAT), Nairobi P.O. Box 62000-00200, Kenya.; Olusegun O. Research Campus, International Institute of Tropical Agriculture (IITA), Bukavu P.O. Box 1222, Democratic Republic of the Congo.; Institut National Pour l'Etude et la Recherche Agronomiques (INERA), Kinshasa P.O. Box 2037, Democratic Republic of the Congo., Kimwemwe PK; Department of Horticulture and Food Security, Jomo Kenyatta University of Agriculture and Technology (JKUAT), Nairobi P.O. Box 62000-00200, Kenya.; Olusegun O. Research Campus, International Institute of Tropical Agriculture (IITA), Bukavu P.O. Box 1222, Democratic Republic of the Congo.; Institut National Pour l'Etude et la Recherche Agronomiques (INERA), Kinshasa P.O. Box 2037, Democratic Republic of the Congo.; Faculty of Agriculture and Environmental Sciences, Université de Kalemie (UNIKAL), Kalemie P.O. Box 570, Democratic Republic of the Congo., Githiri SM; Department of Horticulture and Food Security, Jomo Kenyatta University of Agriculture and Technology (JKUAT), Nairobi P.O. Box 62000-00200, Kenya., Mamati EG; Department of Horticulture and Food Security, Jomo Kenyatta University of Agriculture and Technology (JKUAT), Nairobi P.O. Box 62000-00200, Kenya., Kimani W; International Livestock Research Institute (ILRI), Nairobi P.O. Box 30709-00100, Kenya., Mutai C; International Livestock Research Institute (ILRI), Nairobi P.O. Box 30709-00100, Kenya., Nganga F; International Livestock Research Institute (ILRI), Nairobi P.O. Box 30709-00100, Kenya., Nguezet PD; International Institute of Tropical Agriculture (IITA), Kalemie P.O. Box 570, Democratic Republic of the Congo., Mignouna J; Olusegun O. Research Campus, International Institute of Tropical Agriculture (IITA), Bukavu P.O. Box 1222, Democratic Republic of the Congo., Civava RM; Institut National Pour l'Etude et la Recherche Agronomiques (INERA), Kinshasa P.O. Box 2037, Democratic Republic of the Congo.; Faculty of Agriculture and Environmental Sciences, Université Evangélique en Afrique (UEA), Bukavu P.O. Box 3323, Democratic Republic of the Congo., Fofana M; Olusegun O. Research Campus, International Institute of Tropical Agriculture (IITA), Bukavu P.O. Box 1222, Democratic Republic of the Congo. |
| Abstrakt: |
Micronutrient deficiencies, particularly of iron (Fe) and zinc (Zn), in the diet contribute to health issues and hidden hunger. Enhancing the Fe and Zn content in globally staple food crops like rice is necessary to address food malnutrition. A Genome-Wide Association Study (GWAS) was conducted using 85 diverse rice accessions from the Democratic Republic of Congo (DRC) to identify genomic regions associated with grain Fe and Zn content. The Fe content ranged from 0.95 to 8.68 mg/100 g on a dry weight basis (dwb) while Zn content ranged from 0.87 to 3.8 mg/100 g (dwb). Using MLM and FarmCPU models, we found 10 significant SNPs out of which one SNP on chromosome 11 was associated with the variation in Fe content and one SNP on chromosome 4 was associated with the Zn content, and both were commonly detected by the two models. Candidate genes belonging to transcription regulator activities, including the bZIP family genes and MYB family genes, as well as transporter activities involved in Fe and Zn homeostasis were identified in the vicinity of the SNP markers and selected. The identified SNP markers hold promise for marker-assisted selection in rice breeding programs aimed at enhancing Fe and Zn content in rice. This study provides valuable insights into the genetic factors controlling Fe and Zn uptake and their transport and accumulation in rice, offering opportunities for developing biofortified rice varieties to combat malnutrition among rice consumers. |
