Morphofunctional Changes in Brain and Peripheral Blood in Adult and Aged Wistar Rats with AlCl 3 -Induced Neurodegeneration.

Autor: Sentyabreva AV; Avtsyn Research Institute of Human Morphology of 'Petrovsky National Research Centre of Surgery', 117418 Moscow, Russia.; Research Institute of Molecular and Cellular Medicine, Peoples' Friendship University of Russia (RUDN University), 117198 Moscow, Russia., Miroshnichenko EA; Avtsyn Research Institute of Human Morphology of 'Petrovsky National Research Centre of Surgery', 117418 Moscow, Russia.; Research Institute of Molecular and Cellular Medicine, Peoples' Friendship University of Russia (RUDN University), 117198 Moscow, Russia., Melnikova EA; Avtsyn Research Institute of Human Morphology of 'Petrovsky National Research Centre of Surgery', 117418 Moscow, Russia., Tsvetkov IS; Avtsyn Research Institute of Human Morphology of 'Petrovsky National Research Centre of Surgery', 117418 Moscow, Russia., Kosyreva AM; Avtsyn Research Institute of Human Morphology of 'Petrovsky National Research Centre of Surgery', 117418 Moscow, Russia.; Research Institute of Molecular and Cellular Medicine, Peoples' Friendship University of Russia (RUDN University), 117198 Moscow, Russia.
Jazyk: angličtina
Zdroj: Biomedicines [Biomedicines] 2023 Aug 22; Vol. 11 (9). Date of Electronic Publication: 2023 Aug 22.
DOI: 10.3390/biomedicines11092336
Abstrakt: Background: the general lifespan has been prolonged greatly during the past century, and the incidence of age-associated diseases, including neurodegenerative ones, has increased as well. However, modelling of age-related pathologies is mostly conducted on adult rodents. We studied morphofunctional changes in the brain and peripheral blood of adult Wistar rats in comparison with old Wistar rats to determine age-related physiological changes and differences in adaptive reactions to AlCl 3 exposure.
Methods: the work was performed on adult and old male Wistar rats. The animals consumed a 100 mg/kg solution of AlCl 3 each day for 60 days. Morphological changes of neurons and microglia, mRNA expression levels of pro-inflammatory and anti-inflammatory cytokines, microglia activation markers, amyloid-related proteins, and hallmarks of cellular senescence, monocyte, and lymphocyte subpopulations in the peripheral blood were examined.
Results: old rats showed increasing hyperchromic neurons in the hippocampus; activation of microglia; upregulation of pro-inflammatory cytokines and cellular senescence markers; downregulation of anti-inflammatory cytokines; and Hif-1a and a decrease in B-cells and monocyte in peripheral blood.
Conclusion: compared to young animals, aged rats respond to aluminum exposure with a severe decline of most cells' function and irreversible neuronal loss. Regarding all reported data, neurodegeneration modelling and investigating of factors capable of accelerating or preventing it should be performed in experimental work on aged animals.
Databáze: MEDLINE
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