Extracellular vesicles biogenesis and uptake concepts: A comprehensive guide to studying host-pathogen communication.

Autor: Sabatke B; Graduate Program in Microbiology, Pathology and Parasitology, Federal University of Paraná, Curitiba, Brazil.; EVAHPI - Extracellular Vesicles and Host-Parasite Interactions Research Group, Carlos Chagas Institute (Fiocruz-PR), Curitiba, Brazil., Rossi IV; EVAHPI - Extracellular Vesicles and Host-Parasite Interactions Research Group, Carlos Chagas Institute (Fiocruz-PR), Curitiba, Brazil.; Graduate Program in Cell and Molecular Biology, Federal University of Paraná, Curitiba, Brazil., Sana A; EVAHPI - Extracellular Vesicles and Host-Parasite Interactions Research Group, Carlos Chagas Institute (Fiocruz-PR), Curitiba, Brazil.; Graduate Program in Cell and Molecular Biology, Federal University of Paraná, Curitiba, Brazil., Bonato LB; Graduate Program in Microbiology, Pathology and Parasitology, Federal University of Paraná, Curitiba, Brazil.; EVAHPI - Extracellular Vesicles and Host-Parasite Interactions Research Group, Carlos Chagas Institute (Fiocruz-PR), Curitiba, Brazil., Ramirez MI; EVAHPI - Extracellular Vesicles and Host-Parasite Interactions Research Group, Carlos Chagas Institute (Fiocruz-PR), Curitiba, Brazil.
Jazyk: angličtina
Zdroj: Molecular microbiology [Mol Microbiol] 2024 Nov; Vol. 122 (5), pp. 613-629. Date of Electronic Publication: 2023 Sep 27.
DOI: 10.1111/mmi.15168
Abstrakt: The study of host-pathogen interactions has increased considerably in recent decades. This intercellular communication has been mediated by extracellular vesicles (EVs) that play an important role during the interaction. EVs are particles of lipid bilayer and described in different types of cells, eukaryotic or prokaryotic. Depending on their biogenesis they are described as exosomes (derived from multivesicular bodies) and microvesicles (derived from the plasma membrane). The EVs carry biomolecules, including nucleic acids, lipids, and proteins that can be released or internalized by other cells in different pathways (endocytosis, macropinocytosis, phagocytosis, or membrane fusion) in the process described as uptake. The balance between biogenesis and uptake of EVs could modify physiological and pathophysiological processes of the cell. This review is focusing on the dynamic roles of release and capture of EVs during host-pathogen interaction. We also do a critical analysis of methodologies for obtaining and analyzing EVs. Finally, we draw attention to critical points to be considered in EV biogenesis and uptake studies.
(© 2023 John Wiley & Sons Ltd.)
Databáze: MEDLINE
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