| Autor: |
Galiciolli MEA; Instituto de Pesquisa Pelé Pequeno Príncipe, Avenida Silva Jardim, 1632, Água Verde, Curitiba 80250-200, PR, Brazil.; Faculdades Pequeno Príncipe, Curitiba 80230-020, PR, Brazil., Silva JF; Instituto de Pesquisa Pelé Pequeno Príncipe, Avenida Silva Jardim, 1632, Água Verde, Curitiba 80250-200, PR, Brazil.; Faculdades Pequeno Príncipe, Curitiba 80230-020, PR, Brazil., Prodocimo MM; Departamento de Biologia Celular e Molecular, Universidade Federal do Paraná, Centro Politécnico, Avenida Cel. Francisco H. dos Santos, 100-Jardim das Américas, Curitiba-PR, Curitiba 81531-980, PR, Brazil., Laureano HA; Instituto de Pesquisa Pelé Pequeno Príncipe, Avenida Silva Jardim, 1632, Água Verde, Curitiba 80250-200, PR, Brazil.; Faculdades Pequeno Príncipe, Curitiba 80230-020, PR, Brazil., Calado SLM; Aplysia Assessoria e Consultoria Ltd., Rua Júlia Lacourt Penna, 335, Jd. Camburi, Vitória 29090-210, ES, Brazil., Oliveira CS; Instituto de Pesquisa Pelé Pequeno Príncipe, Avenida Silva Jardim, 1632, Água Verde, Curitiba 80250-200, PR, Brazil.; Faculdades Pequeno Príncipe, Curitiba 80230-020, PR, Brazil., Guiloski IC; Instituto de Pesquisa Pelé Pequeno Príncipe, Avenida Silva Jardim, 1632, Água Verde, Curitiba 80250-200, PR, Brazil.; Faculdades Pequeno Príncipe, Curitiba 80230-020, PR, Brazil. |
| Abstrakt: |
Vaccination programs in the first years of a child's life are effective and extremely important strategies for the successful eradication of diseases. However, as no intervention is without risks, the metal-based components of some vaccines, such as thimerosal (TMS), a preservative composed of ethylmercury, and aluminum (Al), have begun to generate distrust on the part of the population. Therefore, this study evaluated the effects of exposure to thimerosal and aluminum hydroxide (alone or in mixture) on Danio rerio (zebrafish) specimens. The fish were exposed to thimerosal and/or aluminum hydroxide intraperitoneally. The liver, kidney, and brain were removed for a biochemical biomarker analysis, histopathological analysis, and metal quantification. As a result, we observed changes in the activity of the analyzed enzymes (SOD, GST, GPx) in the kidney and brain of the zebrafish, a reduction in GSH levels in all analyzed tissues, and a reduction in MT levels in the kidney and liver as well as in the brain. Changes in AChE enzyme activity were observed. The biochemical results corroborate the changes observed in the lesion index and histomorphology sections. We emphasize the importance of joint research on these compounds to increase the population's safety against their possible toxic effects. |
