| Autor: |
Madalosso LM; Laboratory of Pharmacological and Toxicological Evaluations Applied to Bioactives Molecules-LaftamBio, Federal University of Pampa, Itaqui 97650-000, Brazil., Balok FRM; Programa de Pós-Graduação em Bioquímica, Federal University of Pampa, Uruguaiana 97650-000, Brazil., Bortolotto VC; Programa de Pós-Graduação em Bioquímica, Federal University of Pampa, Uruguaiana 97650-000, Brazil., Dahleh MMM; Programa de Pós-Graduação em Bioquímica, Federal University of Pampa, Uruguaiana 97650-000, Brazil., Backes LG; Laboratory of Pharmacological and Toxicological Evaluations Applied to Bioactives Molecules-LaftamBio, Federal University of Pampa, Itaqui 97650-000, Brazil., Escalante ESS; Laboratory of Pharmacological and Toxicological Evaluations Applied to Bioactives Molecules-LaftamBio, Federal University of Pampa, Itaqui 97650-000, Brazil., Benites FV; Laboratory of Pharmacological and Toxicological Evaluations Applied to Bioactives Molecules-LaftamBio, Federal University of Pampa, Itaqui 97650-000, Brazil., da Silva E Silva FA; Laboratory of Pharmacological and Toxicological Evaluations Applied to Bioactives Molecules-LaftamBio, Federal University of Pampa, Itaqui 97650-000, Brazil., Segat HJ; Laboratory of Pharmacological and Toxicological Evaluations Applied to Bioactives Molecules-LaftamBio, Federal University of Pampa, Itaqui 97650-000, Brazil., Boeira SP; Laboratory of Pharmacological and Toxicological Evaluations Applied to Bioactives Molecules-LaftamBio, Federal University of Pampa, Itaqui 97650-000, Brazil.; Programa de Pós-Graduação em Bioquímica, Federal University of Pampa, Uruguaiana 97650-000, Brazil. |
| Abstrakt: |
Mycotoxins are toxic fungal metabolites and are responsible for contaminating several foods. The intake of foods contaminated by these substances is related to hepatotoxicity and carcinogenic effects, possibly due to increasing oxidative stress. The current study evaluated Pitaya fruit juice's antioxidant effects on oxidative damage aflatoxin B1 (AFB1)-induced. Rats received 1.5 mL of Pitaya juice via gavage (for 30 days), and on the 31st day, they received AFB1 (250 µg/kg, via gavage). Forty-eight hours after the AFB1 dose, rats were euthanized for dosages of alanine transaminase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP); dosage of oxidative markers (thiobarbituric acid reactive species (TBARS), reactive species (RS)) and antioxidant defenses (catalase (CAT), superoxide dismutase (SOD), Glutathione S-transferase (GST) activities and Glutathione (GSH)) levels in the liver; and detection of Heat shock protein 70 (Hsp-70) and nuclear factor- erythroid 2-related factor 2 (Nrf2) immunocontent in the liver. Our results indicated that the Pitaya juice reduced ALP activity. Further, rats exposed to AFB1 experienced liver damage due to the increase in TBARS, RS, and Hsp-70 and the reduction in CAT, GSH, and Nrf2. Pitaya juice could, however, protect against these damages. Finally, these results indicated that pre-treatment with Pitaya juice was effective against the oxidative damage induced. However, other aspects may be elucidated in the future to discover more targets of its action against mycotoxicosis. |
