| Autor: |
Arroyo-Herrera I; Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Prol. de Carpio y Plan de Ayala s/n, Col. Santo Tomás, Del Miguel Hidalgo, Mexico City, 11340, Mexico., Román-Ponce B; Universidad Politécnica del Estado de Morelos. Boulevard Cuauhnáhuac #566, Col. Lomas del Texcal, Jiutepec, Morelos, 62550, Mexico., Bustamante-Brito R; Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Av. Universidad s/n, Colonia Chamilpa, Cuernavaca, Morelos, 62210, Mexico., Guevara-Luna J; Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Prol. de Carpio y Plan de Ayala s/n, Col. Santo Tomás, Del Miguel Hidalgo, Mexico City, 11340, Mexico., Larios-Serrato V; Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Prol. de Carpio y Plan de Ayala s/n, Col. Santo Tomás, Del Miguel Hidalgo, Mexico City, 11340, Mexico., Carro L; Departamento de Microbiología y Genética, Facultad de CC Agrarias y Ambientales, Universidad de Salamanca, Plaza Doctores de la Reina, Lab 230, 37007, Salamanca, Spain., Mariano Igual J; Instituto de Recursos Naturales y Agrobiología de Salamanca, Consejo Superior de Investigaciones Científicas (IRNASA-CSIC), c/Cordel de Merinas 40-52, 37008, Salamanca, Spain., Geiger O; Centro de Ciencias Genómicas, Universidad Nacional Autónoma de México, Av. Universidad s/n, Colonia Chamilpa, Cuernavaca, Morelos, 62210, Mexico., Sánchez-Reyes A; Investigador por México, CONAHCYT-Instituto de Biotecnología, Universidad Nacional Autónoma de México, Av. Universidad 2001, Colonia Chamilpa, Cuernavaca, Morelos, 62209, Mexico., Estrada-de Los Santos P; Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Prol. de Carpio y Plan de Ayala s/n, Col. Santo Tomás, Del Miguel Hidalgo, Mexico City, 11340, Mexico., Wang ET; Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Prol. de Carpio y Plan de Ayala s/n, Col. Santo Tomás, Del Miguel Hidalgo, Mexico City, 11340, Mexico., Vásquez-Murrieta MS; Instituto Politécnico Nacional, Escuela Nacional de Ciencias Biológicas, Prol. de Carpio y Plan de Ayala s/n, Col. Santo Tomás, Del Miguel Hidalgo, Mexico City, 11340, Mexico. |
| Abstrakt: |
Four Gram-positive, aerobic, catalase- and oxidase-negative, rod-shaped, motile endophytic bacterial strains, designated NM3R9 T , NE1TT3, NE2TL11 and NE2HP2 T , were isolated from the inner tissues (leaf and stem) of Sphaeralcea angustifolia and roots of Prosopis laevigata . They were characterized using a polyphasic approach, which revealed that they represent two novel Microbacterium species. Phylogenetic analysis based on 16S rRNA gene sequencing showed that the species closest to NE2HP2 T was Microbacterium arborescens DSM 20754 T (99.6 %) and that closest to NM3R9 T , NE2TL11 and NE2TT3 was Microbacterium oleivorans NBRC 103075 T (97.4 %). The whole-genome average nucleotide identity value between strain NM3R9 T and Microbacterium imperiale DSM 20530 T was 90.91 %, and that between strain NE2HP2 T and M. arborecens DSM 20754 T was 91.03 %. Digital DNA-DNA hybridization showed values of less than 70 % with the type strains of related species. The polar lipids present in both strains included diphosphatidylglycerol, phosphatidylglycerol, glycolipids and unidentified lipids, whereas the major fatty acids included anteiso-C 15 : 0 , anteiso-C 17 : 0 , iso-C 16 : 0 and C 16 : 0 . Whole-cell sugars included mannose, rhamnose and galactose. Strains NM3R9 T and NE2HP2 T showed physiological characteristics different from those present in closely related Microbacterium species. According to the taxonomic analysis, both strains belong to two novel species. The name Microbacterium plantarum sp. nov. is proposed for strain NE2HP2 T (=LMG 30875 T =CCBAU 101117 T ) and Microbacterium thalli sp. nov. for strains NM3R9 T (=LMG 30873 T =CCBAU 101116 T ), NE1TT3 (=CCBAU 101114) and NE2TL11 (=CCBAU 101115). |
