| Autor: |
Paglia EB; School of Chemical Engineering, Department of Process and Product Development, University of Campinas, Campinas 13083-852, Brazil., Baldin EKK; School of Chemical Engineering, Department of Process and Product Development, University of Campinas, Campinas 13083-852, Brazil.; Renato Archer Information Technology Center, Campinas 13069-901, Brazil., Freitas GP; School of Chemical Engineering, Department of Process and Product Development, University of Campinas, Campinas 13083-852, Brazil.; Renato Archer Information Technology Center, Campinas 13069-901, Brazil., Santiago TSA; School of Chemical Engineering, Department of Process and Product Development, University of Campinas, Campinas 13083-852, Brazil., Neto JBMR; Technology Center, Federal University of Alagoas, Maceió 57072-900, Brazil., Silva JVL; Renato Archer Information Technology Center, Campinas 13069-901, Brazil., Carvalho HF; Institute of Biology, Department of Structural and Functional Biology, University of Campinas, Campinas 13083-864, Brazil., Beppu MM; School of Chemical Engineering, Department of Process and Product Development, University of Campinas, Campinas 13083-852, Brazil. |
| Abstrakt: |
The early and non-invasive diagnosis of tumor diseases has been widely investigated by the scientific community focusing on the development of sensors/biomarkers that act as a way of recognizing the adhesion of circulating tumor cells (CTCs). As a challenge in this area, strategies for CTCs capture and enrichment currently require improvements in the sensors/biomarker's selectivity. This can be achieved by understanding the biological recognition factors for different cancer cell lines and also by understanding the interaction between surface parameters and the affinity between macromolecules and the cell surface. To overcome some of these concerns, electrochemical sensors have been used as precise, fast-response, and low-cost transduction platforms for application in cytosensors. Additionally, distinct materials, geometries, and technologies have been investigated to improve the sensitivity and specificity properties of the support electrode that will transform biochemical events into electrical signals. This review identifies novel approaches regarding the application of different specific biomarkers (CD44, Integrins, and EpCAm) for capturing CTCs. These biomarkers can be applied in electrochemical biosensors as a cytodetection strategy for diagnosis of cancerous diseases. |
