Generation, culture, and stimulation of small intestinal murine organoids in parasitology research.
| Autor: | Campillo Poveda M; Wellcome Centre of Integrative Parasitology, School of Infection and Immunity, University of Glasgow, Glasgow, UK. Electronic address: marta.campillopoveda@glasgow.ac.uk., Drurey C; Wellcome Centre of Integrative Parasitology, School of Infection and Immunity, University of Glasgow, Glasgow, UK., Maizels RM; Wellcome Centre of Integrative Parasitology, School of Infection and Immunity, University of Glasgow, Glasgow, UK. Electronic address: rick.maizels@glasgow.ac.uk. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2023 Dec 15; Vol. 4 (4), pp. 102608. Date of Electronic Publication: 2023 Sep 25. |
| DOI: | 10.1016/j.xpro.2023.102608 |
| Abstrakt: | Parasitic helminth worms frequently infect the gastrointestinal tract and interact with the intestinal epithelium and specialized cell types within it. Intestinal organoids derived from stem cells that line the intestine represent a transformational technology in the study of epithelial-parasite dialogue. Here, we present a protocol for establishing small intestine organoid cultures and administering parasite products of interest to these cultures. We then describe steps for evaluating their impact by microscopy, flow cytometry, immunohistology, and mRNA gene expression. For complete details on the use and execution of this protocol, please refer to Drurey et al. (2022). 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Crown Copyright © 2023. Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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