Autor: |
Park S; Department of Life Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea., Kim I; Department of Life Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea., Chhetri G; Department of Life Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea., So Y; Department of Life Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea., Jung Y; Department of Life Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea., Woo H; Department of Life Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea., Seo T; Department of Life Science, Dongguk University-Seoul, Goyang 10326, Republic of Korea. |
Abstrakt: |
Three Gram-stain-negative, facultatively anaerobic, rod-shaped, catalase-positive, oxidase-negative bacterial strains were designated as hw1 T , hw8 T and hw3 T . Strains hw1 T , hw8 T and hw3 T grew at 15-28 °C (optimum, 25 °C), 15-35 °C (optimum, 30 °C) and 4-28 °C (optimum, 20 °C), respectively, and at pH 7.0-12.0 (optimum, pH 9.0), pH 6.0-11.0 (optimum, pH 9.0) and 5.0-12.0 (optimum, pH 7.0), respectively. Additionally, strains hw1 T and hw8 T only grew when the NaCl concentration was 0 %, while strain hw3 T grew at between 0 and 0.5 % (w/v; optimum, 0 %). The average nucleotide identity (ANI) values between strains hw1 T , hw8 T and the Roseateles type strains ranged from 73.8 to 84.2 %, while the digital DNA-DNA hybridization (dDDH) values ranged from 19.7 to 27.5 %. The ANI values between strain hw3 T and the Janthinobacterium type strains ranged from 78.7 to 80.7 %, while dDDH values ranged from 22.3 to 23.0 %. The draft genomes of strains hw1 T , hw8 T and hw3 T consisted of 5.5, 4.4 and 5.9 Mbp, with DNA G+C contents of 61.7, 61.8 and 66.0 mol%, respectively. The results of the dDDH, ANI, phylogenetic, biochemical and physiological analyses indicated that the novel strains were distinct from other members of their genera. Thus, we proposed the names Roseateles albus sp. nov. (type strain hw1 T = KACC 22887 T = TBRC 16613 T ), Roseateles koreensis sp. nov. (type strain hw8 T = KACC 22885 T = TBRC 16614 T ) and Janthinobacterium fluminis sp. nov. (type strain hw3 T = KACC 22886 T = TBRC 16615 T ). |