Comparing Effectiveness of Hyaluronic Acid-Chitosan Nanoparticles Encapsulation Versus Hyaluronic Acid Monotherapy in Osteoarthritis Rat Model: Microarray Screening for miR-140.

Autor:	Kamar SS; Histology Department, Kasr Al-ainy Faculty of Medicine, Cairo University, Cairo 11559, Egypt., ShamsEldeen AM; Physiology Department, Kasr Al-ainy Faculty of Medicine, Cairo University, Cairo 11559, Egypt., Hosny SA; Histology Department, Kasr Al-ainy Faculty of Medicine, Cairo University, Cairo 11559, Egypt., El-Shafei AA; Histology Department, Kasr Al-ainy Faculty of Medicine, Cairo University, Cairo 11559, Egypt., Rashid LA; Biochemistry Department, Kasr Al-ainy Faculty of Medicine, Cairo University, Cairo 11559, Egypt., Hassanein RT; Biochemistry Department, Kasr Al-ainy Faculty of Medicine, Cairo University, Cairo 11559, Egypt., Hassan RM; Histology Department, Kasr Al-ainy Faculty of Medicine, Cairo University, Cairo 11559, Egypt.
Jazyk:	angličtina
Zdroj:	Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada [Microsc Microanal] 2023 Apr 05; Vol. 29 (2), pp. 686-697.
DOI:	10.1093/micmic/ozac048
Abstrakt:	Osteoarthritis is a debilitating, progressive joint disease linked to lower quality of life and higher health care costs. This study compared hyaluronic acid-chitosan nanoparticle encapsulation to hyaluronic-acid monotherapy in a rat model of knee osteoarthritis. Four groups of 40 adult male albino rats were designed. Group (Gp) I: control; Gp II (osteoarthritis model): intra-articular injection of monoiodoacetate; Gp III (hyaluronic acid-treated): intra-articular injections of hyaluronic-acid on days 14 and 21 after monoiodoacetate injection; and Gp IV (hyaluronic acid-chitosan nanoparticle-treated): intra-articular injections of hyaluronic acid-chitosan nanoparticle on days 14 and 21 after monoiodoacetate injection. After 28 days, knee joints were examined using H&E, Safranin O, and immunohistochemistry for nuclear factor kappa beta (NF-κB), inducible nitric oxide synthase (iNOS), and matrix metalloproteinase (MMP)-13. Quantification for gene expression of collagen-II, aggrecan, and micro-RNA-140; ELISA for interleukin (IL)-1β and IL-8; and western blotting for IKBα and NF-κB was estimated. Osteoarthritis-knee joints showed a severe cartilage damage and synovial inflammation with increased NF-κB, iNOS, and MMP-13 immunostaining, decreased miR-140, collagen II, and aggrecan levels, and increased inflammatory markers' gene expressions. The hyaluronic acid-chitosan nanoparticle significantly improved knee joint structure and reduced inflammatory cytokines compared to hyaluronic acid monotherapy. Intra-articular injection of hyaluronic acid-chitosan nanoparticle encapsulation revealed a significant improvement in the knee joint structure compared to hyaluronic-acid in a rat model of osteoarthritis. Competing Interests: Conflict of interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (© The Author(s) 2023. Published by Oxford University Press on behalf of the Microscopy Society of America. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.)
Databáze:	MEDLINE
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