Seromolecular surveillance of rabbit haemorrhagic disease virus in Nigeria.

Autor: Daodu OB; Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Kwara State, Nigeria. daodu.ob@unilorin.edu.ng., Shaibu JO; Center for Human Virology and Genomics, Nigerian Institute for Medical Research, Yaba, Lagos State, Nigeria., Aderounmu EA; Ogun State Ministry of Agriculture and Rural Development, Ogun State, Abeokuta, Nigeria., Jolaoso TO; Ogun State Ministry of Agriculture and Rural Development, Ogun State, Abeokuta, Nigeria., Oluwayelu DO; Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, Oyo State, Nigeria., Akanbi OB; Department of Veterinary Pathology, Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Kwara State, Nigeria., Olorunshola ID; Department of Veterinary Microbiology, Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Kwara State, Nigeria., Aiyedun JO; Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Kwara State, Nigeria., Oludairo OO; Department of Veterinary Public Health and Preventive Medicine, Faculty of Veterinary Medicine, University of Ilorin, Ilorin, Kwara State, Nigeria., Audu RA; Center for Human Virology and Genomics, Nigerian Institute for Medical Research, Yaba, Lagos State, Nigeria., Daodu OC; Department of Wildlife and Ecotourism, Faculty of Agriculture and Forestry, University of Ibadan, Ibadan, Oyo State, Nigeria.
Jazyk: angličtina
Zdroj: Tropical animal health and production [Trop Anim Health Prod] 2023 Sep 26; Vol. 55 (5), pp. 327. Date of Electronic Publication: 2023 Sep 26.
DOI: 10.1007/s11250-023-03753-7
Abstrakt: Following the first 2020 rabbit haemorrhagic disease virus (RHDV) outbreak in Nigeria which caused massive mortalities in several rabbitries, there was a need to know the spread and strains circulating in the affected states. Over 100 rabbitries still existing post-RHDV outbreak in Ogun and Kwara States were investigated. A commercial enzyme-linked immunosorbent assay kit was used to screen for RHDV immunoglobulin G in 192 rabbit sera, while RHDV VP60 gene was amplified in RNA extracted from these sera and tissues (liver and/or spleen harvested from 37 carcasses necrotized) by reverse transcription-polymerase chain reaction (RT-PCR). Sequences obtained from the amplicons were subjected to phylogenetic analysis. The results revealed a seroprevalence of 82.3% (158/192). RHDV VP60 gene was detected in 15/17 (88.2%) and 2/20 (10.0%) carcasses from Ogun and Kwara States, respectively, while none of the sera was positive. Sequences of the two positive amplicons selected (one from each states) shared 98.95% nucleotide identity and belonged to RHDV 2/GI.2 strain. Also, nBLAST of these sequences revealed 98.43-99.55% homology with the prototype Nigerian RHDV strain RHDV/NGR/ILN/001 (MT996357.1). Furthermore, these strains clustered with this prototype and a German RHDV strain (LR899166.1). Pathologic lesions affecting the respiratory, cardiovascular, renal, lymphatic, and digestive systems were observed in necropsied carcasses. This study indicated that RHDV 2/GI.2 strain was the cause of 2020 RHD outbreak in Nigeria. Thus, while continuous public sensitization about RHD especially among rabbit farmers in Nigeria is important, efforts aimed at design and implementation of RHD vaccination policy, preferably using indigenous seed, should be expedited.
(© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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