A Glycolipidated-liposomal peptide vaccine confers long-term mucosal protection against Streptococcus pyogenes via IL-17, macrophages and neutrophils.

Autor: Ozberk V; Institute for Glycomics, Griffith University, Gold Coast, QLD, Australia., Zaman M; Institute for Glycomics, Griffith University, Gold Coast, QLD, Australia., Lepletier A; Institute for Glycomics, Griffith University, Gold Coast, QLD, Australia., Eskandari S; Institute for Glycomics, Griffith University, Gold Coast, QLD, Australia., Kaden J; Institute for Glycomics, Griffith University, Gold Coast, QLD, Australia., Mills JL; Institute for Glycomics, Griffith University, Gold Coast, QLD, Australia., Calcutt A; Institute for Glycomics, Griffith University, Gold Coast, QLD, Australia., Dooley J; Institute for Glycomics, Griffith University, Gold Coast, QLD, Australia., Huo Y; Institute for Glycomics, Griffith University, Gold Coast, QLD, Australia., Langshaw EL; Institute for Glycomics, Griffith University, Gold Coast, QLD, Australia., Ulett GC; School of Pharmacy and Medical Science, and Menzies Health Institute Queensland, Griffith University, Gold Coast, Australia., Batzloff MR; Institute for Glycomics, Griffith University, Gold Coast, QLD, Australia., Good MF; Institute for Glycomics, Griffith University, Gold Coast, QLD, Australia. michael.good@griffith.edu.au., Pandey M; Institute for Glycomics, Griffith University, Gold Coast, QLD, Australia. m.pandey@griffith.edu.au.
Jazyk: angličtina
Zdroj: Nature communications [Nat Commun] 2023 Sep 25; Vol. 14 (1), pp. 5963. Date of Electronic Publication: 2023 Sep 25.
DOI: 10.1038/s41467-023-41410-7
Abstrakt: Mucosally active subunit vaccines are an unmet clinical need due to lack of licensed immunostimulants suitable for vaccine antigens. Here, we show that intranasal administration of liposomes incorporating: the Streptococcus pyogenes peptide antigen, J8; diphtheria toxoid as a source of T cell help; and the immunostimulatory glycolipid, 3D(6-acyl) PHAD (PHAD), is able to induce long-lived humoral and cellular immunity. Mice genetically deficient in either mucosal antibodies or total antibodies are protected against S. pyogenes respiratory tract infection. Utilizing IL-17-deficient mice or depleting cellular subsets using antibodies, shows that the cellular responses encompassing, CD4 + T cells, IL-17, macrophages and neutrophils have important functions in vaccine-mediated mucosal immunity. Overall, these data demonstrate the utility of a mucosal vaccine platform to deliver multi-pronged protective responses against a highly virulent pathogen.
(© 2023. Springer Nature Limited.)
Databáze: MEDLINE
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