Study of the Antioxidant, Antimicrobial, and Wound Healing Properties of Raw Hydrolyzed Extract from Nile Tilapia Skin (Oreochromis niloticus).

Autor: Tozetto R; Department of Pharmaceutical Sciences, State University of Ponta Grossa, General, Carlos Cavalcanti Avenue, 4748, 84900-030, Paraná, Brazil., Santos Rocha B; Department of Chemistry, Federal University of Paraná, Dr. João Maxímiano Street, 426, 86900-000, Paraná, Brazil., Assis de Andrade E; Pharmaceutical Science Post-graduation Program, State University of Ponta Grossa, General Carlos Cavalcanti Avenue, 4748, 84900-030, Paraná, Brazil., Stolz Cruz L; Department of Pharmaceutical Sciences, State University of Centro-Oeste, Élio Antonio Dalla Vecchia Street, 838, 85040-167, Paraná, Brazil., da Rosa RL; Pharmaceutical Science Post-graduation Program, State University of Ponta Grossa, General Carlos Cavalcanti Avenue, 4748, 84900-030, Paraná, Brazil., Machinski I; Pharmaceutical Science Post-graduation Program, State University of Ponta Grossa, General Carlos Cavalcanti Avenue, 4748, 84900-030, Paraná, Brazil., Lemos de Oliveira É; Chemistry Post-graduation Program, State University of Maringá, Colombo Avenue, 5790, 87020-900, Paraná, Brazil., Borges Monteiro JR; Department of Pharmaceutical Sciences, Federal University of Espírito Santo, Fernando Ferrari Avenue, 514, 29075-910, Espírito Santo, Brazil., Koga AY; Department of Medicine, State University of Ponta Grossa, General Carlos Cavalcanti Avenue, 4748, 84900-030, Paraná, Brazil., Cavalcante Lipinski L; Department of Medicine, State University of Ponta Grossa, General Carlos Cavalcanti Avenue, 4748, 84900-030, Paraná, Brazil., Meurer EC; Department of Chemistry, Federal University of Paraná, Dr. João Maxímiano Street, 426, 86900-000, Paraná, Brazil., Kitagawa RR; Department of Pharmaceutical Sciences, Federal University of Espírito Santo, Fernando Ferrari Avenue, 514, 29075-910, Espírito Santo, Brazil., Beltrame FL; Department of Pharmaceutical Sciences, State University of Ponta Grossa, General, Carlos Cavalcanti Avenue, 4748, 84900-030, Paraná, Brazil.; Pharmaceutical Science Post-graduation Program, State University of Ponta Grossa, General Carlos Cavalcanti Avenue, 4748, 84900-030, Paraná, Brazil.
Jazyk: angličtina
Zdroj: Chemistry & biodiversity [Chem Biodivers] 2023 Nov; Vol. 20 (11), pp. e202300863. Date of Electronic Publication: 2023 Oct 12.
DOI: 10.1002/cbdv.202300863
Abstrakt: Oreochromis niloticus (Nile tilapia) skin is a by-product of Brazilian fish farming, rich in collagen. The present study aims to evaluate the wound healing, antioxidant, and antimicrobial potential of the raw hydrolyzed extract of Nile tilapia skin, as well as the identification of the main compounds. The in vitro activity was performed using antioxidant, antimicrobial and scratch wound healing assays. An in vivo experiment was performed to evaluate the wound healing potential. On days 1, 7, 14 and 21, the lesions were photographed to assess wound retraction and on the 7 th , 14 th and 21 st  days the skins were removed for histological evaluation and the blood of the animals was collected for glutamic oxaloacetic transaminase and glutamic pyruvic transaminase determination. The chemical study was carried out through liquid chromatography-tandem mass spectrometry and de novo sequencing of peptides. The in vitro assays showed a reduction of the gap area in 24 h, dose-dependent antimicrobial activity for both bacteria, and antioxidant activity. The chemical analysis highlighted the presence of active biopeptides. The histological evaluation showed that the raw hydrolyzed extract of Nile tilapia skin has a healing potential, and does not present toxicological effects; therefore, is promising for the treatment of wounds.
(© 2023 Wiley-VHCA AG, Zurich, Switzerland.)
Databáze: MEDLINE
Externí odkaz: