| Autor: |
Wolf J; Molecular Surgery Laboratory, Stanford University; Department of Ophthalmology, Byers Eye Institute, Stanford University., Chemudupati T; Molecular Surgery Laboratory, Stanford University; Department of Ophthalmology, Byers Eye Institute, Stanford University., Kumar A; Molecular Surgery Laboratory, Stanford University; Department of Ophthalmology, Byers Eye Institute, Stanford University., Rasmussen DK; Molecular Surgery Laboratory, Stanford University; Department of Ophthalmology, Byers Eye Institute, Stanford University., Wai KM; Department of Ophthalmology, Byers Eye Institute, Stanford University., Chang RT; Department of Ophthalmology, Byers Eye Institute, Stanford University., Montague AA; Department of Ophthalmology, Byers Eye Institute, Stanford University., Tang PH; Department of Ophthalmology and Visual Neurosciences, University of Minnesota; Retina Consultants of Minnesota., Bassuk AG; Department of Pediatrics, University of Iowa; Department of Neurology, University of Iowa; The Iowa Neuroscience Institute (INI), University of Iowa., Dufour A; Department of Physiology and Pharmacology, Cumming School of Medicine, University of Calgary; Department of Biochemistry and Molecular Biology, Cumming School of Medicine, University of Calgary., Mruthrunjaya P; Department of Ophthalmology, Byers Eye Institute, Stanford University., Mahajan VB; Molecular Surgery Laboratory, Stanford University; Department of Ophthalmology, Byers Eye Institute, Stanford University; Veterans Affairs Palo Alto Health Care System; vinit.mahajan@standford.edu. |
| Abstrakt: |
A critical challenge in translational research is establishing a viable and efficient interface between patient care in the operating room (OR) and the research laboratory. Here, we developed a protocol for acquiring high-quality liquid biopsies for molecular analyses from the aqueous humor and the vitreous from patients undergoing eye surgery. In this workflow, a Mobile Operating Room Lab Interface (MORLI) cart equipped with a computer, a barcode scanner, and lab instruments, including onboard cold storage, is used to obtain and archive human biological samples. A web-based data privacy-compliant database enables annotating each sample over its lifetime, and a cartesian coordinate system allows tracking each barcoded specimen in storage, enabling quick and accurate retrieval of samples for downstream analyses. Molecular characterization of human tissue samples not only serves as a diagnostic tool (e.g., to distinguish between infectious endophthalmitis and other non-infectious intraocular inflammation) but also represents an important component of translational research, allowing the identification of new drug targets, development of new diagnostic tools, and personalized therapeutics. |
