A mechanosensing mechanism controls plasma membrane shape homeostasis at the nanoscale.

Autor:	Quiroga X; Institute for Bioengineering of Catalonia, the Barcelona Institute of Technology (BIST), Barcelona, Spain.; Departament de Biomedicina, Unitat de Biofísica i Bioenginyeria, Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona, Barcelona, Spain., Walani N; Department of Applied Mechanics, IIT Delhi, New Delhi, India., Disanza A; IFOM ETS - The AIRC Institute of Molecular Oncology, Milan, Italy., Chavero A; Departament de Biomedicina, Unitat de Biologia Cel·lular, Facultat de Medicina i Ciències de la Salut, Centre de Recerca Biomèdica CELLEX, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Universitat de Barcelona, Barcelona, Spain., Mittens A; Institute for Bioengineering of Catalonia, the Barcelona Institute of Technology (BIST), Barcelona, Spain., Tebar F; Departament de Biomedicina, Unitat de Biologia Cel·lular, Facultat de Medicina i Ciències de la Salut, Centre de Recerca Biomèdica CELLEX, Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Universitat de Barcelona, Barcelona, Spain., Trepat X; Institute for Bioengineering of Catalonia, the Barcelona Institute of Technology (BIST), Barcelona, Spain., Parton RG; Institute for Molecular Bioscience and Centre for Microscopy and Microanalysis, University of Queensland, Brisbane, Australia., Geli MI; Institute for Molecular Biology of Barcelona (CSIC), Barcelona, Spain., Scita G; IFOM ETS - The AIRC Institute of Molecular Oncology, Milan, Italy.; Department of Oncology and Haemato-Oncology, University of Milan, Milan, Italy., Arroyo M; Institute for Bioengineering of Catalonia, the Barcelona Institute of Technology (BIST), Barcelona, Spain.; Universitat Politècnica de Catalunya (UPC), Campus Nord, Carrer de Jordi Girona, Barcelona, Spain.; Centre Internacional de Mètodes Numèrics en Enginyeria (CIMNE), Barcelona, Spain., Le Roux AL; Institute for Bioengineering of Catalonia, the Barcelona Institute of Technology (BIST), Barcelona, Spain., Roca-Cusachs P; Institute for Bioengineering of Catalonia, the Barcelona Institute of Technology (BIST), Barcelona, Spain.; Departament de Biomedicina, Unitat de Biofísica i Bioenginyeria, Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona, Barcelona, Spain.
Jazyk:	angličtina
Zdroj:	ELife [Elife] 2023 Sep 25; Vol. 12. Date of Electronic Publication: 2023 Sep 25.
DOI:	10.7554/eLife.72316
Abstrakt:	As cells migrate and experience forces from their surroundings, they constantly undergo mechanical deformations which reshape their plasma membrane (PM). To maintain homeostasis, cells need to detect and restore such changes, not only in terms of overall PM area and tension as previously described, but also in terms of local, nanoscale topography. Here, we describe a novel phenomenon, by which cells sense and restore mechanically induced PM nanoscale deformations. We show that cell stretch and subsequent compression reshape the PM in a way that generates local membrane evaginations in the 100 nm scale. These evaginations are recognized by I-BAR proteins, which triggers a burst of actin polymerization mediated by Rac1 and Arp2/3. The actin polymerization burst subsequently re-flattens the evagination, completing the mechanochemical feedback loop. Our results demonstrate a new mechanosensing mechanism for PM shape homeostasis, with potential applicability in different physiological scenarios. Competing Interests: XQ, NW, AD, AC, AM, FT, XT, RP, GS, MA, AL, PR No competing interests declared, MG Reviewing editor, eLife (© 2023, Quiroga et al.)
Databáze:	MEDLINE
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