Developmental Mouse Brain Common Coordinate Framework.
Autor: | Kronman FA; Department of Neural and Behavioral Sciences, College of Medicine, The Pennsylvania State University, Hershey, PA., Liwang JK; Department of Neural and Behavioral Sciences, College of Medicine, The Pennsylvania State University, Hershey, PA., Betty R; Department of Neural and Behavioral Sciences, College of Medicine, The Pennsylvania State University, Hershey, PA., Vanselow DJ; Department of Neural and Behavioral Sciences, College of Medicine, The Pennsylvania State University, Hershey, PA., Wu YT; Department of Neural and Behavioral Sciences, College of Medicine, The Pennsylvania State University, Hershey, PA., Tustison NJ; Department of Radiology and Medical Imaging, University of Virginia, Charlottesville, VA., Bhandiwad A; Allen Institute for Brain Science, Seattle, WA, USA., Manjila SB; Department of Neural and Behavioral Sciences, College of Medicine, The Pennsylvania State University, Hershey, PA., Minteer JA; Department of Neural and Behavioral Sciences, College of Medicine, The Pennsylvania State University, Hershey, PA., Shin D; Department of Neural and Behavioral Sciences, College of Medicine, The Pennsylvania State University, Hershey, PA., Lee CH; Bernard and Irene Schwartz Center for Biomedical Imaging, Department of Radiology, New York University School of Medicine, NY, USA., Patil R; Department of Neural and Behavioral Sciences, College of Medicine, The Pennsylvania State University, Hershey, PA., Duda JT; Department of Radiology, Penn Image Computing and Science Lab, University of Pennsylvania, Philadelphia, Pennsylvania., Puelles L; Department of Human Anatomy and Psychobiology, Faculty of Medicine, Universidad de Murcia, and Murcia Arrixaca Institute for Biomedical Research (IMIB) Murcia, Spain., Gee JC; Department of Radiology, Penn Image Computing and Science Lab, University of Pennsylvania, Philadelphia, Pennsylvania., Zhang J; Bernard and Irene Schwartz Center for Biomedical Imaging, Department of Radiology, New York University School of Medicine, NY, USA., Ng L; Allen Institute for Brain Science, Seattle, WA, USA., Kim Y; Department of Neural and Behavioral Sciences, College of Medicine, The Pennsylvania State University, Hershey, PA. |
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Jazyk: | angličtina |
Zdroj: | BioRxiv : the preprint server for biology [bioRxiv] 2023 Sep 15. Date of Electronic Publication: 2023 Sep 15. |
DOI: | 10.1101/2023.09.14.557789 |
Abstrakt: | 3D standard reference brains serve as key resources to understand the spatial organization of the brain and promote interoperability across different studies. However, unlike the adult mouse brain, the lack of standard 3D reference atlases for developing mouse brains has hindered advancement of our understanding of brain development. Here, we present a multimodal 3D developmental common coordinate framework (DevCCF) spanning mouse embryonic day (E) 11.5, E13.5, E15.5, E18.5, and postnatal day (P) 4, P14, and P56 with anatomical segmentations defined by a developmental ontology. At each age, the DevCCF features undistorted morphologically averaged atlas templates created from Magnetic Resonance Imaging and co-registered high-resolution templates from light sheet fluorescence microscopy. Expert-curated 3D anatomical segmentations at each age adhere to an updated prosomeric model and can be explored via an interactive 3D web-visualizer. As a use case, we employed the DevCCF to unveil the emergence of GABAergic neurons in embryonic brains. Moreover, we integrated the Allen CCFv3 into the P56 template with stereotaxic coordinates and mapped spatial transcriptome cell-type data with the developmental ontology. In summary, the DevCCF is an openly accessible resource that can be used for large-scale data integration to gain a comprehensive understanding of brain development. Competing Interests: Conflict of Interest We declare no conflict of interest. |
Databáze: | MEDLINE |
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