| Autor: |
Ramanathan R; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520 USA.; Microbial Sciences Institute, Yale University, West Haven, CT 06516 USA., Hatzios SK; Department of Molecular, Cellular, and Developmental Biology, Yale University, New Haven, CT 06520 USA.; Microbial Sciences Institute, Yale University, West Haven, CT 06516 USA.; Department of Chemistry, Yale University, New Haven, CT 06520 USA. |
| Abstrakt: |
Host cells sense and respond to pathogens by dynamically regulating cell signaling. The rapid modulation of signaling pathways is achieved by post-translational modifications (PTMs) that can alter protein structure, function, and/or binding interactions. By using chemical probes to broadly profile changes in enzyme function or side-chain reactivity, activity-based protein profiling (ABPP) can reveal PTMs that regulate host-microbe interactions. While ABPP has been widely utilized to uncover microbial mechanisms of pathogenesis, in this review, we focus on more recent applications of this technique to the discovery of host PTMs and enzymes that modulate signaling within infected cells. Collectively, these advances underscore the importance of ABPP as a tool for interrogating the host response to infection and identifying potential targets for host-directed therapies. |
