A Glycosylphosphatidylinositol-Anchored α-Amylase Encoded by amyD Contributes to a Decrease in the Molecular Mass of Cell Wall α-1,3-Glucan in Aspergillus nidulans .

Autor:	Miyazawa K; Laboratory of Applied Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.; Laboratory of Filamentous Mycoses, Department of Fungal Infection, National Institute of Infectious Diseases, Tokyo, Japan., Yamashita T; Laboratory of Applied Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan., Takeuchi A; Laboratory of Applied Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan., Kamachi Y; Laboratory of Applied Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan., Yoshimi A; Laboratory of Environmental Interface Technology of Filamentous Fungi, Graduate School of Agriculture, Kyoto University, Kyoto, Japan.; ABE-Project, New Industry Creation Hatchery Center, Tohoku University, Sendai, Japan., Tashiro Y; Laboratory of Applied Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan., Koizumi A; Laboratory of Applied Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan., Ogata M; Faculty of Food and Agricultural Sciences, Fukushima University, Fukushima, Japan., Yano S; Department of Biochemical Engineering, Graduate School of Engineering, Yamagata University, Yonezawa, Japan., Kasahara S; Food Microbiology Unit, School of Food and Agricultural Sciences, Miyagi University, Sendai, Japan., Sano M; Genome Biotechnology Laboratory, Kanazawa Institute of Technology, Hakusan, Japan., Yamagata Y; Department of Applied Life Science, The United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, Japan., Nakajima T; ABE-Project, New Industry Creation Hatchery Center, Tohoku University, Sendai, Japan., Abe K; Laboratory of Applied Microbiology, Department of Microbial Biotechnology, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.; ABE-Project, New Industry Creation Hatchery Center, Tohoku University, Sendai, Japan.; Department of Microbial Resources, Graduate School of Agricultural Science, Tohoku University, Sendai, Japan.
Jazyk:	angličtina
Zdroj:	Frontiers in fungal biology [Front Fungal Biol] 2022 Jan 28; Vol. 2, pp. 821946. Date of Electronic Publication: 2022 Jan 28 (Print Publication: 2021).
DOI:	10.3389/ffunb.2021.821946
Abstrakt:	α-1,3-Glucan is one of the main polysaccharides in the cell wall of Aspergillus nidulans . We previously revealed that it plays a role in hyphal aggregation in liquid culture, and that its molecular mass (MM) in an agsA -overexpressing ( agsA OE ) strain was larger than that in an agsB -overexpressing ( agsB OE ) strain. The mechanism that regulates its MM is poorly understood. Although the gene amyD , which encodes glycosylphosphatidylinositol (GPI)-anchored α-amylase (AmyD), is involved in the biosynthesis of α-1,3-glucan in A . nidulans , how it regulates this biosynthesis remains unclear. Here we constructed strains with disrupted amyD (Δ amyD ) or overexpressed amyD ( amyD OE ) in the genetic background of the ABPU1 (wild-type), agsA OE , or agsB OE strain, and characterized the chemical structure of α-1,3-glucans in the cell wall of each strain, focusing on their MM. The MM of α-1,3-glucan from the agsB OE amyD OE strain was smaller than that in the parental agsB OE strain. In addition, the MM of α-1,3-glucan from the agsA OE Δ amyD strain was greater than that in the agsA OE strain. These results suggest that AmyD is involved in decreasing the MM of α-1,3-glucan. We also found that the C-terminal GPI-anchoring region is important for these functions. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2022 Miyazawa, Yamashita, Takeuchi, Kamachi, Yoshimi, Tashiro, Koizumi, Ogata, Yano, Kasahara, Sano, Yamagata, Nakajima and Abe.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu