Protocol for multispectral imaging on cryosections to map myeloid cell heterogeneity in its spatial context.

Autor:	Wieland EB; Cardiovascular Research Institute Maastricht, Experimental Vascular Pathology, Department of Pathology, Maastricht University Medical Center+, Maastricht, The Netherlands., Kempen LJAP; Cardiovascular Research Institute Maastricht, Experimental Vascular Pathology, Department of Pathology, Maastricht University Medical Center+, Maastricht, The Netherlands; Laboratory of Immunology and Vaccinology, Faculty of Veterinary Medicine, FARAH, Liège University, Liège, Belgium; Laboratory of Immunophysiology, GIGA Institute, Liège University, Liège, Belgium., Lu C; Cardiovascular Research Institute Maastricht, Experimental Vascular Pathology, Department of Pathology, Maastricht University Medical Center+, Maastricht, The Netherlands; Institute for Computational Biomedicine, Heidelberg, Germany., Donners MMPC; Cardiovascular Research Institute Maastricht, Experimental Vascular Pathology, Department of Pathology, Maastricht University Medical Center+, Maastricht, The Netherlands., Biessen EAL; Cardiovascular Research Institute Maastricht, Experimental Vascular Pathology, Department of Pathology, Maastricht University Medical Center+, Maastricht, The Netherlands; Institute for Molecular Cardiovascular Research, RWTH Aachen University, Aachen, Germany., Goossens P; Cardiovascular Research Institute Maastricht, Experimental Vascular Pathology, Department of Pathology, Maastricht University Medical Center+, Maastricht, The Netherlands. Electronic address: pieter.goossens@maastrichtuniversity.nl.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2023 Dec 15; Vol. 4 (4), pp. 102601. Date of Electronic Publication: 2023 Sep 23.
DOI:	10.1016/j.xpro.2023.102601
Abstrakt:	Recent technical advances, such as single-cell RNA sequencing and mass cytometry, improve identification of cell types and subsets in a range of healthy and diseased tissues at the expense of their cellular and molecular context. Here, we present a protocol for in situ multispectral imaging to map myeloid cell heterogeneity in tissue cryosections, describing steps for cutting sequential sections, antibody titration, and building a spectral library. We then detail procedures for multispectral imaging and preparing data for downstream analysis. For complete details on the use and execution of this protocol, please refer to Goossens et al. (2022). 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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