Naringin improves post-ischemic myocardial injury by activation of K ATP channels.

Autor: Araujo AM; Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil., Cerqueira SVS; Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil., Menezes-Filho JER; Department of Biochemistry and Immunology, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil., Heimfarth L; Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil., Matos KKOG; Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil., Mota KO; Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil., Conceição MRL; Department of Biophysics, Federal University of São Paulo, São Paulo, Brazil., Marques LP; Department of Biophysics, Federal University of São Paulo, São Paulo, Brazil., Roman-Campos D; Department of Biophysics, Federal University of São Paulo, São Paulo, Brazil., Santos-Neto AGD; Institute of Technology and Research, Tiradentes University, Aracaju, Sergipe, Brazil., Albuquerque-Júnior RLC; Department of Pathology, Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil., Santos VCO; Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil., Vasconcelos CML; Department of Physiology, Federal University of Sergipe, São Cristóvão, Sergipe, Brazil. Electronic address: carlamlv@hotmail.com.
Jazyk: angličtina
Zdroj: European journal of pharmacology [Eur J Pharmacol] 2023 Nov 05; Vol. 958, pp. 176069. Date of Electronic Publication: 2023 Sep 22.
DOI: 10.1016/j.ejphar.2023.176069
Abstrakt: Naringin (NRG) is a flavonoid with recognized cardioprotective effects. Then, it was investigated the cardioprotective mechanisms of NRG against ischemia-reperfusion (I/R) injury. The rats were pretreated for 7 days (v.o.) with NRG (25 mg/kg) or n-acetylcysteine (NAC, 100 mg/kg) and their isolated hearts were subjected to global ischemia (30 min) and reperfusion (60 min). Furthermore, isolated hearts were perfused with 5 μM NRG in the presence of 10 μM glibenclamide (GLI) and subjected to I/R protocol. In healthy ventricular cardiomyocyte, it was evaluated the acute effect of 5 μM NRG on the GLI sensitive current. The results showed that NRG pretreatment restored the cardiac function and electrocardiogram (ECG) alterations induced by I/R injury, decreasing arrhythmia scores and the occurrence of severe arrhythmias. Lactate dehydrogenase and infarct area were decreased while superoxide dismutase (SOD), catalase and citrate synthase activities increased. Expression of SOD CuZn and SOD Mn not was altered. NRG treatment decreased reactive oxygen species (ROS) generation and lipid peroxidation without alter sulfhydryl groups and protein carbonylation. Also, NRG (5 μM) increased the glibenclamide sensitive current in isolated cardiomyocytes. In isolated heart, the cardioprotection of NRG was significantly reduced by GLI. Furthermore, NRG promoted downregulation of Bax expression and Bax/Bcl-2. Histopathological analysis showed that NRG decreased cell edema, cardiomyocytes and nucleus diameter. Thus, NRG has a cardioprotective effect against cardiac I/R injury which is mediated by its antioxidant and antiapoptotic actions and K ATP channels activation.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Carla Maria Lins de Vasconcelos reports was provided by Federal University of Sergipe. Carla Maria Lins de Vasconcelos reports a relationship with Federal University of Sergipe that includes: non-financial support. The authors declare no conflicts of interest.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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