Recovery of extracorporeal lungs using cross-circulation with injured recipient swine.

Autor:	Chen P; Department of Biomedical Engineering, Columbia University, New York, NY; Department of Surgery, Columbia University Medical Center, New York, NY., Van Hassel J; Department of Biomedical Engineering, Columbia University, New York, NY; Department of Surgery, Columbia University Medical Center, New York, NY., Pinezich MR; Department of Biomedical Engineering, Columbia University, New York, NY., Diane M; Department of Biomedical Engineering, Columbia University, New York, NY., Hudock MR; Department of Biomedical Engineering, Columbia University, New York, NY., Kaslow SR; Department of Biomedical Engineering, Columbia University, New York, NY; Department of Surgery, Columbia University Medical Center, New York, NY., Gavaudan OP; Department of Biomedical Engineering, Columbia University, New York, NY., Fung K; Clinical Perfusion, Columbia University Medical Center, New York, NY., Kain ML; Institute of Comparative Medicine, Columbia University, New York, NY., Lopez H 2nd; Clinical Perfusion, Columbia University Medical Center, New York, NY., Saqi A; Pathology and Cell Biology, Columbia University Medical Center, New York, NY., Guenthart BA; Department of Cardiothoracic Surgery, Stanford University Medical Center, Stanford, Calif., Hozain AE; Department of Surgery, Columbia University Medical Center, New York, NY., Romanov A; Institute of Comparative Medicine, Columbia University, New York, NY., Bacchetta M; Department of Cardiac Surgery, Vanderbilt University Medical Center, Nashville, Tenn; Department of Biomedical Engineering, Vanderbilt University, Nashville, Tenn., Vunjak-Novakovic G; Department of Biomedical Engineering, Columbia University, New York, NY. Electronic address: Gv2131@columbia.edu.
Jazyk:	angličtina
Zdroj:	The Journal of thoracic and cardiovascular surgery [J Thorac Cardiovasc Surg] 2024 May; Vol. 167 (5), pp. e106-e130. Date of Electronic Publication: 2023 Sep 21.
DOI:	10.1016/j.jtcvs.2023.09.032
Abstrakt:	Objective: Lung transplantation remains limited by the shortage of healthy organs. Cross-circulation with a healthy swine recipient provides a durable physiologic environment to recover injured donor lungs. In a clinical application, a recipient awaiting lung transplantation could be placed on cross-circulation to recover damaged donor lungs, enabling eventual transplantation. Our objective was to assess the ability of recipient swine with respiratory compromise to tolerate cross-circulation and support recovery of donor lungs subjected to extended cold ischemia. Methods: Swine donor lungs (n = 6) were stored at 4 °C for 24 hours while recipient swine (n = 6) underwent gastric aspiration injury before cross-circulation. Longitudinal multiscale analyses (blood gas, bronchoscopy, radiography, histopathology, cytokine quantification) were performed to evaluate recipient swine and extracorporeal lungs on cross-circulation. Results: Recipient swine lung injury resulted in sustained, impaired oxygenation (arterial oxygen tension/inspired oxygen fraction ratio 205 ± 39 mm Hg vs 454 ± 111 mm Hg at baseline). Radiographic, bronchoscopic, and histologic assessments demonstrated bilateral infiltrates, airway cytokine elevation, and significantly worsened lung injury scores. Recipient swine provided sufficient metabolic support for extracorporeal lungs to demonstrate robust functional improvement (0 hours, arterial oxygen tension/inspired oxygen fraction ratio 138 ± 28.2 mm Hg; 24 hours, 539 ± 156 mm Hg). Multiscale analyses demonstrated improved gross appearance, aeration, and cellular regeneration in extracorporeal lungs by 24 hours. Conclusions: We demonstrate that acutely injured recipient swine tolerate cross-circulation and enable recovery of donor lungs subjected to extended cold storage. This proof-of-concept study supports feasibility of cross-circulation for recipients with isolated lung disease who are candidates for this clinical application. (Copyright © 2023 The American Association for Thoracic Surgery. Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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