CARBOHYDRATES METABOLISM IN THE BLOOD OF RATS WITH IMPAIRED GLUCOSE TOLERANCE UNDER LONG TERM MELATONIN INJECTIONS.

Autor:	Kushnir OY; BUKOVYNIAN STATE MEDICAL UNIVERSITY, CHERNIVTSI, UKRAINE., Yaremii IM; BUKOVYNIAN STATE MEDICAL UNIVERSITY, CHERNIVTSI, UKRAINE., Pantsiuk KA; VITA CLINIC OF SPECIALISTS AND DENTISTS, WROCŁAW, POLAND., Vivsyannuk VV; BUKOVYNIAN STATE MEDICAL UNIVERSITY, CHERNIVTSI, UKRAINE., Tymkul DM; BUKOVYNIAN STATE MEDICAL UNIVERSITY, CHERNIVTSI, UKRAINE., Vlasova KV; BUKOVYNIAN STATE MEDICAL UNIVERSITY, CHERNIVTSI, UKRAINE., Vlasova OV; BUKOVYNIAN STATE MEDICAL UNIVERSITY, CHERNIVTSI, UKRAINE.
Jazyk:	angličtina
Zdroj:	Wiadomosci lekarskie (Warsaw, Poland : 1960) [Wiad Lek] 2023; Vol. 76 (8), pp. 1761-1767.
DOI:	10.36740/WLek202308109
Abstrakt:	Objective: The aim: To determine the influence of melatonin on the glucose level and content of malondialdehyde, activities of pyruvate kinase and glucose-6-phosphate dehydrogenase enzymes in the blood; histochemical features of glycogen distribution in liver of rats with impaired glucose tolerance. Patients and Methods: Materials and methods: Diabetes in rats was induced by intra-abdominal injection of a 5% solution of alloxan monohydrate at the rate of 170 mg/kg of body weight. Four days after animals were divided into rats with impaired glucose tolerance and melatonin-group with impaired glucose tolerance (5 mg/ kg «Sigma» USA, daily and intraperitoneal for 42 days starting from 5th day). Impaired glucose tolerance was determined by measurement of glucose profiles - fasting <5.6 mmol/l; postprandial (2h post-load) 7.8 - 11.0 mmol/l. Histochemical examination of the liver was performed according to the standard method of PAS-reaction staining. Statistical analysis was performed using Statistica 10 StatSoft Inc. Results: Results: Pyruvate kinase activity in erythrocytes and optical density of glycogen in hepatocytes of animals with impaired glucose tolerance decreased on 18% and 11%, activity of glucose-6-phosphate dehydrogenase and content of malondialdehyde increased on 35% and 23%, respectively compared with the control. We have reached the recovery of the pyruvate kinase and normalization of glucose-6-phosphate dehydrogenase activities, malondialdehyde levels, glucose profiles in the blood as well as glycogen distribution in the liver caused by melatonin injections. Conclusion: Conclusions: We have determined that long term melatonin injections did better glucose tolerance in rats.
Databáze:	MEDLINE
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