Protocol for screening facultative parthenogenesis in Drosophila.
| Autor: | Sperling AL; Department of Genetics, University of Cambridge, Cambridge, CB2 3EH Cambridgeshire, UK. Electronic address: alb84@cam.ac.uk., Glover DM; Department of Genetics, University of Cambridge, Cambridge, CB2 3EH Cambridgeshire, UK; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA. Electronic address: dmglover@caltech.edu. |
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| Jazyk: | angličtina |
| Zdroj: | STAR protocols [STAR Protoc] 2023 Dec 15; Vol. 4 (4), pp. 102585. Date of Electronic Publication: 2023 Sep 22. |
| DOI: | 10.1016/j.xpro.2023.102585 |
| Abstrakt: | Most species of sexually reproducing Drosophila are capable of some degree of facultative parthenogenesis, which involves the initiation of development in an unfertilized egg. Here, we present an optimized protocol to screen facultative parthenogenesis in Drosophila. We describe steps for the collection and maintenance of virgin flies. We then detail offspring screening for the analysis of parthenogenesis. This protocol can be applied to different Drosophila strains and can be adapted for the analysis of parthenogenesis in other animals. For complete details on the use and execution of this protocol, please refer to Sperling et al. 1 . Competing Interests: Declaration of interests The authors declare no competing interests. (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.) |
| Databáze: | MEDLINE |
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