Adipose-derived stem cells significantly increases collagen level and fiber maturity in patient-specific biological engineered blood vessels.

Autor: Wonski BT; Department of Biomedical Engineering, Wayne State University, Detroit, Michigan, United States of America., Patel B; Department of Biomedical Engineering, Wayne State University, Detroit, Michigan, United States of America., Tepper DG; Department of Plastic and Reconstructive Surgery, Henry Ford Health System, Detroit, Michigan, United States of America., Siddiqui A; Department of Plastic and Reconstructive Surgery, Henry Ford Health System, Detroit, Michigan, United States of America., Kabbani LS; Department of Vascular Surgery, Henry Ford Health System, Detroit, Michigan, United States of America., Lam MT; Department of Biomedical Engineering, Wayne State University, Detroit, Michigan, United States of America.
Jazyk: angličtina
Zdroj: PloS one [PLoS One] 2023 Sep 22; Vol. 18 (9), pp. e0291766. Date of Electronic Publication: 2023 Sep 22 (Print Publication: 2023).
DOI: 10.1371/journal.pone.0291766
Abstrakt: Tissue engineering has driven significant research in the strive to create a supply of tissues for patient treatment. Cell integration into engineered tissues maximizes functional capabilities, however, issues of rejection remain. Autologous cell sources able to solve this issue are difficult to identify for tissue engineering purposes. Here, we present the efficacy of patient-sourced cells derived from adipose (adipose-derived stem cells, ASCs) and skin tissue (dermal fibroblasts, PtFibs) to build a combined engineered tunica media and adventitia graft, respectively. Patient cells were integrated into our lab's vascular tissue engineering technique of forming vascular rings that are stacked into a tubular structure to create the vascular graft. For the media layer, ASCs were successfully differentiated into the smooth muscle phenotype using angiotensin II followed by culture in smooth muscle growth factors, evidenced by significantly increased expression of αSMA and myosin light chain kinase. Engineered media vessels composed of differentiated ASCs (ASC-SMCs) exhibited an elastic modulus (45.2 ± 18.9 kPa) between that of vessels of undifferentiated ASCs (71.8 ± 35.3 kPa) and control human aortic smooth muscle cells (HASMCs; 18.7 ± 5.49 kPa) (p<0.5). Tensile strength of vessels composed of ASCs (41.3 ± 15.7 kPa) and ASC-SMCs (37.3 ± 17.0 kPa) were higher compared to vessels of HASMCs (28.4 ± 11.2 kPa). ASC-based tissues exhibited a significant increase in collagen content and fiber maturity- both factors contribute to tissue strength and stability. Furthermore, vessels gained stability and a more-uniform single-tubular shape with longer-term 1-month culture. This work demonstrates efficacy of ASCs and PtFibs to create patient-specific vessels.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2023 Wonski et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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