Photo-Cross-Linkable, Injectable, and Highly Adhesive GelMA-Glycol Chitosan Hydrogels for Cartilage Repair.

Autor: Paul S; Centre for Biomedical Technologies, Queensland University of Technology, 60 Musk Ave., Kelvin Grove, QLD, 4059, Australia.; Department of Surgery and Radiology, Faculty of Veterinary Medicine and Animal Science, Bangabandhu Sheikh Mujibur Rahman Agricultural University (BSMRAU), Gazipur, 1706, Bangladesh.; School of Mechanical, Medical and Process Engineering, Queensland University of Technology (QUT), 2 George Street, Brisbane, QLD, 4000, Australia., Schrobback K; School of Biomedical Sciences, Centre for Genomics and Personalised Health, Translational Research Institute, Queensland University of Technology (QUT), 37 Kent Street, Woolloongabba, QLD, 4102, Australia., Tran PA; Centre for Biomedical Technologies, Queensland University of Technology, 60 Musk Ave., Kelvin Grove, QLD, 4059, Australia.; School of Mechanical, Medical and Process Engineering, Queensland University of Technology (QUT), 2 George Street, Brisbane, QLD, 4000, Australia., Meinert C; Centre for Biomedical Technologies, Queensland University of Technology, 60 Musk Ave., Kelvin Grove, QLD, 4059, Australia.; School of Mechanical, Medical and Process Engineering, Queensland University of Technology (QUT), 2 George Street, Brisbane, QLD, 4000, Australia.; Chief Executive Officer of Gelomics Pty Ltd, Brisbane, Queensland, 4059, Australia., Davern JW; Centre for Biomedical Technologies, Queensland University of Technology, 60 Musk Ave., Kelvin Grove, QLD, 4059, Australia.; School of Mechanical, Medical and Process Engineering, Queensland University of Technology (QUT), 2 George Street, Brisbane, QLD, 4000, Australia.; ARC Training Centre for Cell and Tissue Engineering Technologies, Queensland University of Technology (QUT), Brisbane, QLD, 4059, Australia., Weekes A; Centre for Biomedical Technologies, Queensland University of Technology, 60 Musk Ave., Kelvin Grove, QLD, 4059, Australia.; School of Mechanical, Medical and Process Engineering, Queensland University of Technology (QUT), 2 George Street, Brisbane, QLD, 4000, Australia., Klein TJ; Centre for Biomedical Technologies, Queensland University of Technology, 60 Musk Ave., Kelvin Grove, QLD, 4059, Australia.; School of Mechanical, Medical and Process Engineering, Queensland University of Technology (QUT), 2 George Street, Brisbane, QLD, 4000, Australia.
Jazyk: angličtina
Zdroj: Advanced healthcare materials [Adv Healthc Mater] 2023 Dec; Vol. 12 (32), pp. e2302078. Date of Electronic Publication: 2023 Oct 10.
DOI: 10.1002/adhm.202302078
Abstrakt: Hydrogels provide a promising platform for cartilage repair and regeneration. Although hydrogels have shown some efficacy, they still have shortcomings including poor mechanical properties and suboptimal integration with surrounding cartilage. Herein, hydrogels that are injectable, cytocompatible, mechanically robust, and highly adhesive to cartilage are developed. This approach uses GelMA-glycol chitosan (GelMA-GC) that is crosslinkable with visible light and photoinitiators (lithium acylphosphinate and tris (2,2'-bipyridyl) dichlororuthenium (II) hexahydrate ([RuII(bpy) 3 ] 2+ and sodium persulfate (Ru/SPS)). Ru/SPS-cross-linked hydrogels have higher compressive and tensile modulus, and most prominently higher adhesive strength with cartilage, which also depends on inclusion of GC. Tensile and push-out tests of the Ru/SPS-cross-linked GelMA-GC hydrogels demonstrate adhesive strength of ≈100 and 46 kPa, respectively. Hydrogel precursor solutions behave in a Newtonian manner and are injectable. After injection in focal bovine cartilage defects and in situ cross-linking, this hydrogel system remains intact and integrated with cartilage following joint manipulation ex vivo. Cells remain viable (>85%) in the hydrogel system and further show tissue regeneration potential after three weeks of in vitro culture. These preliminary results provide further motivation for future research on bioadhesive hydrogels for cartilage repair and regeneration.
(© 2023 The Authors. Advanced Healthcare Materials published by Wiley-VCH GmbH.)
Databáze: MEDLINE
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