Human gut epithelium features recapitulated in MINERVA 2.0 millifluidic organ-on-a-chip device.

Autor:	Donnaloja F; Department of Chemistry, Materials and Chemical Engineering 'Giulio Natta,' Politecnico di Milano, Milan, Italy., Izzo L; Department of Chemistry, Materials and Chemical Engineering 'Giulio Natta,' Politecnico di Milano, Milan, Italy., Campanile M; Department of Chemistry, Materials and Chemical Engineering 'Giulio Natta,' Politecnico di Milano, Milan, Italy., Perottoni S; Department of Chemistry, Materials and Chemical Engineering 'Giulio Natta,' Politecnico di Milano, Milan, Italy., Boeri L; Department of Chemistry, Materials and Chemical Engineering 'Giulio Natta,' Politecnico di Milano, Milan, Italy., Fanizza F; Department of Chemistry, Materials and Chemical Engineering 'Giulio Natta,' Politecnico di Milano, Milan, Italy., Sardelli L; Department of Chemistry, Materials and Chemical Engineering 'Giulio Natta,' Politecnico di Milano, Milan, Italy., Jacchetti E; Department of Chemistry, Materials and Chemical Engineering 'Giulio Natta,' Politecnico di Milano, Milan, Italy., Raimondi MT; Department of Chemistry, Materials and Chemical Engineering 'Giulio Natta,' Politecnico di Milano, Milan, Italy., Rito LD; Department of Oncology, Computational Oncology Unit, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Milan, Italy., Craparotta I; Department of Oncology, Computational Oncology Unit, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Milan, Italy., Bolis M; Department of Oncology, Computational Oncology Unit, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Milan, Italy., Giordano C; Department of Chemistry, Materials and Chemical Engineering 'Giulio Natta,' Politecnico di Milano, Milan, Italy., Albani D; Department of Neuroscience, Istituto di Ricerche Farmacologiche Mario Negri IRCCS, Milan, Italy.
Jazyk:	angličtina
Zdroj:	APL bioengineering [APL Bioeng] 2023 Sep 19; Vol. 7 (3), pp. 036117. Date of Electronic Publication: 2023 Sep 19 (Print Publication: 2023).
DOI:	10.1063/5.0144862
Abstrakt:	We developed an innovative millifluidic organ-on-a-chip device, named MINERVA 2.0, that is optically accessible and suitable to serial connection. In the present work, we evaluated MINERVA 2.0 as millifluidic gut epithelium-on-a-chip by using computational modeling and biological assessment. We also tested MINERVA 2.0 in a serially connected configuration prodromal to address the complexity of multiorgan interaction. Once cultured under perfusion in our device, human gut immortalized Caco-2 epithelial cells were able to survive at least up to 7 days and form a three-dimensional layer with detectable tight junctions (occludin and zonulin-1 positive). Functional layer development was supported by measurable trans-epithelial resistance and FITC-dextran permeability regulation, together with mucin-2 expression. The dynamic culturing led to a specific transcriptomic profile, assessed by RNASeq, with a total of 524 dysregulated transcripts (191 upregulated and 333 downregulated) between static and dynamic condition. Overall, the collected results suggest that our gut-on-a-chip millifluidic model displays key gut epithelium features and, thanks to its modular design, may be the basis to build a customizable multiorgan-on-a-chip platform. Competing Interests: The authors have no conflicts to disclose. (© 2023 Author(s).)
Databáze:	MEDLINE
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