Efficient malachite green biodegradation by Pseudomonas plecoglossicide MG2: process optimization, application in bioreactors, and degradation pathway.

Autor: El-Bendary MA; Microbial Chemistry Department, Biotechnology Research Institute, National Research Centre, 33 Bohouth St., Dokki, Giza, Egypt. tasnim41@yahoo.com., Fawzy ME; Water Pollution Research Department, Environmental Research and Climate Change Institute, National Research Centre, 33 Bohouth st., Dokki, Giza, Egypt., Abdelraof M; Microbial Chemistry Department, Biotechnology Research Institute, National Research Centre, 33 Bohouth St., Dokki, Giza, Egypt., El-Sedik M; Dyeing, Printing and Textile Auxiliaries Department, Textile Research and Technology Institute, National Research Centre, 33 Bohouth st., Dokki, Giza, Egypt., Allam MA; Spectroscopy Department, Physics Research Institute, National Research Centre, 33 Bohouth st., Dokki, Giza, Egypt.
Jazyk: angličtina
Zdroj: Microbial cell factories [Microb Cell Fact] 2023 Sep 21; Vol. 22 (1), pp. 192. Date of Electronic Publication: 2023 Sep 21.
DOI: 10.1186/s12934-023-02194-z
Abstrakt: Microbial degradation of synthetic dyes is considered a promising green dye detoxification, cost-effective and eco-friendly approach. A detailed study on the decolorization and degradation of malachite green dye (MG) using a newly isolated Pseudomonas plecoglossicide MG2 was carried out. Optimization of MG biodegradation by the tested organism was investigated by using a UV-Vis spectrophotometer and the resultant degraded products were analyzed by liquid chromatography-mass spectrometry and FTIR. Also, the cytotoxicity of MG degraded products was studied on a human normal retina cell line. The optimum conditions for the significant maximum decolorization of MG dye (90-93%) by the tested organism were pH 6-7, inoculum size 4-6%, and incubation temperature 30-35 °C, under static and aerobic conditions. The performance of Pseudomonas plecoglossicide MG2 grown culture in the bioreactors using simulated wastewater was assessed. MG degradation (99% at 100 and 150 mg MG/l at an optimal pH) and COD removal (95.95%) by using Pseudomonas plecoglossicide MG2 culture were the best in the tested culture bioreactor in comparison with that in activated sludge or tested culture-activated sludge bioreactors.The FTIR spectrum of the biodegraded MG displayed significant spectral changes, especially in the fingerprint region 1500-500 as well as disappearance of some peaks and appearance of new peaks. Twelve degradation intermediates were identified by LC-MS. They were desmalachite green, didesmalachite green, tetradesmalachite green, 4-(diphenylmethyl)aniline, malachite green carbinol, bis[4-(dimethylamino)phenyl]methanone, [4-(dimethylamino)phenyl][4-(methyl-amino)phenyl]methanone, bis[4-(methylamino)phenyl]methanone, (4-amino- phenyl)[4-(methylamino)phenyl]methanone, bis(4-amino phenyl)methanone, (4-amino phenyl)methanone, and 4-(dimathylamino)benzaldehyde. According to LC-MS and FTIR data, two pathways for MG degradation by using Pseudomonas plecoglossicide MG2 were proposed. MG showed cytotoxicity to human normal retina cell line with LC 50 of 28.9 µg/ml and LC 90 at 79.7 µg/ml. On the other hand, MG bio-degraded products showed no toxicity to the tested cell line. Finally, this study proved that Pseudomonas plecoglossicide MG2 could be used as an efficient, renewable, eco-friendly, sustainable and cost-effective biotechnology tool for the treatment of dye wastewater effluent.
(© 2023. BioMed Central Ltd., part of Springer Nature.)
Databáze: MEDLINE
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