Fibroblasts Derived From Vestibular Schwannoma Express Protumorogenic Markers.

Autor: Baruah P, Marshall J; Institute of Inflammation and Ageing, University of Birmingham, Birmingham, UK., Jones PN; Institute of Inflammation and Ageing, University of Birmingham, Birmingham, UK., Major T; Institute of Inflammation and Ageing, University of Birmingham, Birmingham, UK., Pucino V; Institute of Inflammation and Ageing, University of Birmingham, Birmingham, UK., O'Neil JD; Institute of Inflammation and Ageing, University of Birmingham, Birmingham, UK., Nefla M; Institute of Inflammation and Ageing, University of Birmingham, Birmingham, UK., McGettrick H; Institute of Inflammation and Ageing, University of Birmingham, Birmingham, UK., Monksfield P; Department of ENT, University Hospitals of Birmingham NHS Trust, Birmingham., Irving R; Department of ENT, University Hospitals of Birmingham NHS Trust, Birmingham., Buckley CD; Institute of Inflammation and Ageing, University of Birmingham, Birmingham, UK.
Jazyk: angličtina
Zdroj: Otology & neurotology : official publication of the American Otological Society, American Neurotology Society [and] European Academy of Otology and Neurotology [Otol Neurotol] 2023 Dec 01; Vol. 44 (10), pp. e755-e765. Date of Electronic Publication: 2023 Sep 15.
DOI: 10.1097/MAO.0000000000004011
Abstrakt: Background and Aim: Vestibular schwannomas (VSs), despite being histologically benign, cause significant morbidity because of their challenging intracranial location and the propensity for growth. The role of the stroma and particularly fibroblasts, in the progression of VS, is not completely understood. This study examines the profile of fibroblasts in VS.
Methods: Seventeen patients undergoing surgical excision of VS were recruited into the study. Reverse transcription with quantitative polymerase chain reaction (RT-qPCR) was performed on VS tissue samples and fibroblast-associated molecules examined. Immunofluorescence and immunohistochemistry in VS tissue were used to study the expression of fibroblast markers CD90 and podoplanin in situ. Fibroblast cultures were established from VS, and RT-qPCR analysis was performed on a panel of fibroblast markers on VS and control tissue fibroblasts.
Results: Several fibroblast-associated molecules including members of galectin family and matrix metalloproteinases were found to be expressed in VS tissue on RT-qPCR analysis. In situ, expression of CD90 and podoplanin was observed in VS tissue both on immunohistochemistry and immunofluorescence. RT-qPCR analysis of fibroblasts from VS and control vestibular neuroepithelium (NE) showed a higher expression of several molecules of the galectin and matrix metalloproteinases family on VS fibroblasts compared with NE fibroblasts.
Conclusion: This work examines fibroblasts from VS and shows qualitative differences from NE fibroblasts on RT-qPCR. Further understanding of the fibroblast function in the progression of VS will potentially unveil new targets to manage VS growth.
Competing Interests: The authors disclose no conflicts of interest.
(Copyright © 2023, Otology & Neurotology, Inc.)
Databáze: MEDLINE