NAC domain transcription factors VNI2 and ATAF2 form protein complexes and regulate leaf senescence.

Autor: Nagahage ISP; Graduate School of Science and Engineering Saitama University Saitama Japan.; Department of Plant Physiology Umeå University Umeå Sweden., Matsuda K; Graduate School of Science and Technology Nara Institute of Science and Technology Ikoma Japan., Miyashita K; Bioproduction Research Institute National Institute of Advanced Industrial Science and Technology (AIST) Tsukuba Japan., Fujiwara S; Bioproduction Research Institute National Institute of Advanced Industrial Science and Technology (AIST) Tsukuba Japan., Mannapperuma C; Umeå Plant Science Centre, Department of Plant Physiology Umeå University Umeå Sweden., Yamada T; Graduate School of Science and Engineering Saitama University Saitama Japan., Sakamoto S; Bioproduction Research Institute National Institute of Advanced Industrial Science and Technology (AIST) Tsukuba Japan.; Global Zero-Emission Research Center National Institute of Advanced Industrial Science and Technology (AIST) Tsukuba Japan., Ishikawa T; Graduate School of Science and Engineering Saitama University Saitama Japan., Nagano M; Graduate School of Science and Engineering Saitama University Saitama Japan.; Present address: College of Life Sciences Ritsumeikan University Kusatsu Japan., Ohtani M; Graduate School of Science and Technology Nara Institute of Science and Technology Ikoma Japan.; Present address: Department of Integrated Biosciences, Graduate School of Frontier Sciences The University of Tokyo Kashiwa Japan., Kato K; Graduate School of Science and Technology Nara Institute of Science and Technology Ikoma Japan., Uchimiya H; Institute for Environmental Science and Technology Saitama University Saitama Japan., Mitsuda N; Bioproduction Research Institute National Institute of Advanced Industrial Science and Technology (AIST) Tsukuba Japan.; Global Zero-Emission Research Center National Institute of Advanced Industrial Science and Technology (AIST) Tsukuba Japan., Kawai-Yamada M; Graduate School of Science and Engineering Saitama University Saitama Japan., Demura T; Graduate School of Science and Technology Nara Institute of Science and Technology Ikoma Japan., Yamaguchi M; Graduate School of Science and Engineering Saitama University Saitama Japan.
Jazyk: angličtina
Zdroj: Plant direct [Plant Direct] 2023 Sep 18; Vol. 7 (9), pp. e529. Date of Electronic Publication: 2023 Sep 18 (Print Publication: 2023).
DOI: 10.1002/pld3.529
Abstrakt: The NAM, ATAF1/2, and CUC2 (NAC) domain transcription factor VND-INTERACTING2 (VNI2) negatively regulates xylem vessel formation by interacting with another NAC domain transcription factor, VASCULAR-RELATED NAC-DOMAIN7 (VND7), a master regulator of xylem vessel formation. Here, we screened interacting proteins with VNI2 using yeast two-hybrid assay and isolated two NAC domain transcription factors, Arabidopsis thaliana ACTIVATION FACTOR 2 (ATAF2) and NAC DOMAIN CONTAINING PROTEIN 102 (ANAC102). A transient gene expression assay showed that ATAF2 upregulates the expression of genes involved in leaf senescence, and VNI2 effectively inhibits the transcriptional activation activity of ATAF2. vni2 mutants accelerate leaf senescence, whereas ataf2 mutants delay leaf senescence. In addition, the accelerated leaf senescence phenotype of the vni2 mutant is recovered by simultaneous mutation of ATAF2 . Our findings strongly suggest that VNI2 interacts with and inhibits ATAF2, resulting in negatively regulating leaf senescence.
Competing Interests: The authors declare that they have no known competing interests that could have appeared to influence this work.
(© 2023 The Authors. Plant Direct published by American Society of Plant Biologists and the Society for Experimental Biology and John Wiley & Sons Ltd.)
Databáze: MEDLINE
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