Tetrahydrobiopterin from cyanide-degrading bacterium Bacillus pumilus strain SVD06 induces apoptosis in human lung adenocarcinoma cell (A549).

Autor: Mahendran R; Cancer Therapeutics Laboratory, Department of Microbial Biotechnology, Bharathiar University, Coimbatore, Tamil Nadu, India.; Cardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan., Selvaraj SP; Molecular and Biological Agricultural Science Program, Taiwan International Graduate Program, Academia Sinica, Taipei, Taiwan.; Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan., Dhanapal AR; Institute of Forest Genetics and Tree Breeding (IFGTB), Forest Campus, Coimbatore, Tamil Nadu, India., Sarasa SB; Cancer Therapeutics Laboratory, Department of Microbial Biotechnology, Bharathiar University, Coimbatore, Tamil Nadu, India., Mathias BM; Computational Science Laboratory, MCC-MRF Innovation Park, Madras Christian College, Chennai, Tamil Nadu, India., Thankappan B; Cancer Therapeutics Laboratory, Department of Microbial Biotechnology, Bharathiar University, Coimbatore, Tamil Nadu, India., Femil Selta DR; Department of Biochemistry and Cancer Research Center, FASCM, Karpagam Academy of Higher Education, Coimbatore, Tamil Nadu, India., Naveen P; Department of Chemistry, Sona College of Arts and Science, Salem, Tamil Nadu, India., Poorani R; Gayatri Vidya parishad Institute of Health Care and Medical Technology, Visakhapatnam, India., Sundhar N; Graduate Institute of Biomedical Sciences, School of Medicine, China Medical University, Taichung, Taiwan., Pillai MM; Tissue Engineering Laboratory, PSG Institute of Advanced Studies, Coimbatore, Tamil Nadu, India., Selvakumar R; Tissue Engineering Laboratory, PSG Institute of Advanced Studies, Coimbatore, Tamil Nadu, India., Huang CY; Cardiovascular and Mitochondrial Related Disease Research Center, Hualien Tzu Chi Hospital, Buddhist Tzu Chi Medical Foundation, Hualien, Taiwan.; Department of Biological Science and Technology, College of Life Sciences, China Medical University, Taichung, Taiwan.; PhD Program for Biotechnology Industry, China Medical University, Taichung, Taiwan.; Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan.; Department of Biotechnology, Asia University, Taichung, Taiwan.; Center of General Education, Buddhist Tzu Chi Medical Foundation, Tzu Chi University of Science and Technology, Hualien, Taiwan., Eswaran R; Department of Zoology, The Madura College, Madurai, Tamil Nadu, India., Angayarkanni J; Cancer Therapeutics Laboratory, Department of Microbial Biotechnology, Bharathiar University, Coimbatore, Tamil Nadu, India.
Jazyk: angličtina
Zdroj: Biotechnology and applied biochemistry [Biotechnol Appl Biochem] 2023 Dec; Vol. 70 (6), pp. 2052-2068. Date of Electronic Publication: 2023 Sep 20.
DOI: 10.1002/bab.2509
Abstrakt: Tetrahydrobiopterin (BH4) is an essential biological cofactor and a derivative of pterin which is considered potent anticancer agents. In continuation of our previous study on the identification of BH4 from cyanide-degrading Bacillus pumilus, the present study focuses on evaluating the anticancer properties of BH4 on A549, a human lung adenocarcinoma. Anticancer activity analysis shows that BH4 inhibited A549 cell growth after 24 h of incubation with 0.02 mg/mL. In acridine orange/ethidium bromide staining, BH4-treated A549 cells showed apoptotic morphology. BH4 treatment caused cell cycle arrest at G0/G1 phase compared to control cells. BH4 augmented p53 expression in alveolar cancer cells by downregulating MDM2 levels. There was downregulation of casp-3 and upregulation of iNOS gene in BH4-treated A549 cells. Further, docking studies indicated that BH4 had significant interactions with the above proteins affirming the apoptosis mechanism. Thus, BH4 could be considered a potential anticancer drug.
(© 2023 International Union of Biochemistry and Molecular Biology, Inc.)
Databáze: MEDLINE
Externí odkaz: