| Autor: |
Gong Y; Department of Biology, Stanford University, Stanford, CA 94305, USA., Dale R; Donald Danforth Plant Science Center, St. Louis, MO 63132 USA., Fung HF; Department of Biology, Stanford University, Stanford, CA 94305, USA., Amador GO; Department of Developmental Biology, Stanford University, Stanford, CA 94305, USA., Smit ME; Department of Biology, Stanford University, Stanford, CA 94305, USA.; Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA., Bergmann DC; Department of Biology, Stanford University, Stanford, CA 94305, USA.; Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA. |
| Abstrakt: |
How flexible developmental programs integrate information from internal and external factors to modulate stem cell behavior is a fundamental question in developmental biology. Cells of the Arabidopsis stomatal lineage modify the balance of stem cell proliferation and differentiation to adjust the size and cell type composition of mature leaves. Here, we report that meristemoids, one type of stomatal lineage stem cell, trigger the transition from asymmetric self-renewing divisions to commitment and terminal differentiation by crossing a critical cell size threshold. Through computational simulation, we demonstrate that this cell size-mediated transition allows robust, yet flexible termination of stem cell proliferation, and we observe adjustments in the number of divisions before the differentiation threshold under several genetic manipulations. We experimentally evaluate several mechanisms for cell size sensing, and our data suggest that this stomatal lineage transition is dependent on a nuclear factor that is sensitive to DNA content. |
