The role of TNF-α and NFkβ in an experimental model of intestinal carcinogenesis with 1,2-dimethyhydrazine.

Autor:	Barreira MA; Universidade Federal do Ceará - Walter Cantídio University Hospital - Fortaleza (Ceará) - Brazil., Campelo MWS; Universidade Federal do Ceará - School of Medicine - Department of Surgery - Fortaleza (Ceará) - Brazil., Rebouças CDSM; Universidade Federal do Ceará - School of Medicine - Department of Morphology - Fortaleza (Ceará) - Brazil., Campelo APBS; Centro Universitário Christus - School of Medicine - Fortaleza (Ceará) - Brazil., Vasconcelos PRL; Universidade Federal do Ceará - School of Medicine - Department of Surgery - Fortaleza (Ceará) - Brazil.
Jazyk:	angličtina
Zdroj:	Acta cirurgica brasileira [Acta Cir Bras] 2023 Sep 18; Vol. 38, pp. e383623. Date of Electronic Publication: 2023 Sep 18 (Print Publication: 2023).
DOI:	10.1590/acb383623
Abstrakt:	Purpose: To analyze the potential of tumor necrosis factor-α (TNF-α) and factor nuclear kappa B (NF-κB) as colorectal cancer (CRC) biomarkers in an experimental model of intestinal carcinogenesis with 1,2-dimethyhydrazine (1,2-DMH). Methods: Twenty-four male Wistar rats were divided into two groups: sham and 1,2-DMH. First, 1,2-DMH (20 mg/kg/week) was administered for 15 consecutive weeks. In the 25th week, proctocolectomy was conducted. Histopathological analysis, immunohistochemistry, and gene expression of TNF-α and NF-κB were performed. Statistical analysis was performed using GraphPad Prism. The location of aberrant crypt foci (ACF) was analyzed by Kruskal-Wallis' test. For analyses with two groups with parametric data, the t-test was used; for non-parametric data, the Mann-Whitney's test was used. P < 0.05 was considered significant. Results: The number of ACF and macroscopic lesions was significantly higher (p < 0.5) in the 1,2-DMH group compared to the sham group, and most ACF were concentrated in the distal segment of the colon. There was a statistically significant increase (p < 0.5) in protein and gene expression of TNF-α and NF-κB in the 1,2-DMH group compared to the sham group. Conclusions: Our results provide supportive evidence that TNF-α and NF-κB pathways are strongly involved in CRC development in rats and might be used as early biomarkers of CRC pathogenesis in experimental studies.
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu