| Autor: |
Sarva K; Central Research Laboratory, Meenakshi Academy of Higher Education and Research (Deemed to be University), Chennai, India., Marimuthu Ragavan R; Department of Microbiology and Biotechnology, Presidency College (Autonomous), Chennai, India., Tadi LJ; All India Institute of Medical Sciences, Bibinagar, Telangana, India., Raja S; All India Institute of Medical Sciences, Bibinagar, Telangana, India., Narasingam A; Central Research Laboratory, Meenakshi Academy of Higher Education and Research (Deemed to be University), Chennai, India. |
| Jazyk: |
angličtina |
| Zdroj: |
Microbial drug resistance (Larchmont, N.Y.) [Microb Drug Resist] 2023 Nov; Vol. 29 (11), pp. 504-509. Date of Electronic Publication: 2023 Sep 20. |
| DOI: |
10.1089/mdr.2023.0040 |
| Abstrakt: |
Infections caused by carbapenem-resistant Klebsiella pneumoniae (CRKP) are a major threat to public health. Timely detection of CRKP will help treat patients with appropriate antibiotics. This study aimed to evaluate the performance of the carbapenemase Nordmann-Poirel (CarbaNP), modified carbapenem inactivation (mCIM), and EDTA carbapenem inactivation (eCIM) methods for the detection of CRKP. We compared the results of the three assays with that of real-time PCR. In total, 195 K. pneumoniae isolates, including 150 carbapenem-resistant and 45 carbapenem-susceptible isolates, were investigated. Carbapenem-resistance genes, such as bla KPC , bla NDM , bla VIM , bla IMP , and bla OXA-48-like , were identified using real-time PCR. Among the 150 CRKP isolates, 94 (62.7%) were positive for bla NDM , 29 (19.3%) were positive for bla OXA-48-like , and 27 (18%) were positive for both bla NDM and bla OXA-48-like . For detecting CRKP isolates, CarbaNP, mCIM, and eCIM showed 96.0%, 95.4%, and 96.7% sensitivity, respectively, and all three methods showed 100% specificity. All three phenotypic confirmatory tests are reliable for identifying CRKP, easy to perform, cost-effective, and can be incorporated with routine antibiotic susceptibility testing. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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