Predictions from a mathematical approach to model ionic signaling for rapid responses of Sertoli cells exhibit similarities to pharmacological approaches.

Autor:	Taques BOM; Department of Electronics, Federal Institute of Santa Catarina (IFSC), Rua Pavão, 1377, CEP: 89220-618, Joinville, SC, Brazil.; Federal University of Technology - Paraná (UTFPR), Av. Sete de Setembro, 3165, CEP: 80230-901 Curitiba, PR, Brazil.; Institute of Biomedical Engineering, Federal University of Santa Catarina (UFSC), Campus Universitário, Trindade, CEP: 88040-900 - Florianópolis, SC, Brazil., Gamba HR; Federal University of Technology - Paraná (UTFPR), Av. Sete de Setembro, 3165, CEP: 80230-901 Curitiba, PR, Brazil., Menegaz D; Departament of Biochemistry. Center of Biological Sciences, Federal University of Santa Catarina. Rua João Pio Duarte Silva 241, Sala G301 - terceiro andar. Córrego Grande. CEP: 88037-9000 - Florianópolis, SC, Brazil.; Cell Bioelectricity Center (NUBIOCEL). Center of Biological Sciences, Federal University of Santa Catarina. Rua João Pio Duarte Silva 241, Sala G301 - terceiro andar. Córrego Grande. CEP: 88037-9000 - Florianópolis, SC, Brazil., Silva FRMB; Departament of Biochemistry. Center of Biological Sciences, Federal University of Santa Catarina. Rua João Pio Duarte Silva 241, Sala G301 - terceiro andar. Córrego Grande. CEP: 88037-9000 - Florianópolis, SC, Brazil.; Cell Bioelectricity Center (NUBIOCEL). Center of Biological Sciences, Federal University of Santa Catarina. Rua João Pio Duarte Silva 241, Sala G301 - terceiro andar. Córrego Grande. CEP: 88037-9000 - Florianópolis, SC, Brazil., Suzuki DOH; Institute of Biomedical Engineering, Federal University of Santa Catarina (UFSC), Campus Universitário, Trindade, CEP: 88040-900 - Florianópolis, SC, Brazil.
Jazyk:	angličtina
Zdroj:	Biomedical physics & engineering express [Biomed Phys Eng Express] 2023 Oct 02; Vol. 9 (6). Date of Electronic Publication: 2023 Oct 02.
DOI:	10.1088/2057-1976/acfb07
Abstrakt:	Sertoli cells are essential for the male reproduction system as they provide morphological support and nutrients for germ cells to guarantee ongoing spermatogenesis. The aim of this work was to predict the electrical properties at the plasma membrane that trigger Sertoli cell rapid responses by involving ionic channels. The rapid responses of Sertoli cells in culture were monitored using patch clamp electrical measurement and compared to data obtained using pharmacological tools (from intact seminiferous tubules). A mathematical model was used to define the roles of potassium channels and the ATP-dependent Na + /K + pump in these responses. Mathematical data verification was also performed to determine the resting and hormonal stimulated membrane potentials of Sertoli cells in the intact seminiferous tubules and of Sertoli cells in culture (patch clamp measurements). The prediction of these data based on mathematical modeling demonstrated, for the first time, the involvement of potassium channels and the activation of Na + /K + pump in the hyperpolarization of Sertoli cells and their consequent rapid responses. Moreover, the mathematical analysis showing the involvement of ionic balance in the rapid responses of these cells to hormones, such as follicle-stimulating hormone, is consistent with previous reports obtained using pharmacological techniques in Sertoli cells. Thus, the validation of such data is reliable and represents a first step in the proposition for a mathematical model to predict rapid responses of Sertoli cells to hormonal stimuli. (© 2023 IOP Publishing Ltd.)
Databáze:	MEDLINE
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