| Autor: |
Sant' Anna CC; Molecular Biology Laboratory, Ophir Loyola Hospital, Belém, Brazil.; Nucleus of Research in Oncology, Hospital Universitário João de Barros Barreto, Federal University of Pará, Belém, Brazil., Migone SRDC; Molecular Biology Laboratory, Ophir Loyola Hospital, Belém, Brazil., Rocha CAMD; Department of Biology and Health Sciences, Federal Institute of Pará, Belém, Brazil., Mello Júnior FAR; Molecular Biology Laboratory, Ophir Loyola Hospital, Belém, Brazil., Seabra AD; Molecular Biology Laboratory, Ophir Loyola Hospital, Belém, Brazil., Pontes TB; Molecular Biology Laboratory, Ophir Loyola Hospital, Belém, Brazil., Rodrigues JM; Molecular Biology Laboratory, Ophir Loyola Hospital, Belém, Brazil., Soares SA; Molecular Biology Laboratory, Ophir Loyola Hospital, Belém, Brazil., Rego VP; Molecular Biology Laboratory, Ophir Loyola Hospital, Belém, Brazil., Figueira JP; Molecular Biology Laboratory, Ophir Loyola Hospital, Belém, Brazil., Rodrigues APM; Molecular Biology Laboratory, Ophir Loyola Hospital, Belém, Brazil., Burbano RMR; Molecular Biology Laboratory, Ophir Loyola Hospital, Belém, Brazil.; Nucleus of Research in Oncology, Hospital Universitário João de Barros Barreto, Federal University of Pará, Belém, Brazil. |
| Abstrakt: |
Cytomegalovirus (CMV) mutations associated with antiviral resistance have become a major problem related to high mortality in kidney transplant patients. The aim of the study was to investigate mutations in the CMV genes UL97 and UL54 associated with antiviral resistance. A retrospective observational cohort study was carried out at Hospital Ophir Loyola (HOL), a reference in Kidney Transplantation. A total of 81 patients who underwent kidney transplantation were followed up between 2016 and 2018 were monitored for CMV viral load by performing qPCR. Sanger sequencing was performed on 66 patients. All CMV-positive kidney transplant recipients were included. Mutations were observed in 15 samples (22.72%) from patients. Most cases involved UL97 mutations. Mutation in UL54 without mutation in UL97 was detected in only 2 cases. Resistance mutations in UL97 were identified, such as M460V, L595S, H520Q, two co-mutations D465R + Del524 and A594P + D413A and a 3 codon deletion (del598-601). The search for mutations in the CMV genes identified mutations that confer resistance to conventional antivirals, such as ganciclovir and cidofovir, used in the treatment of these patients. Confirmation of the association with increased CMV viral load in transplanted patients, due to mutation in resistance genes, requires phenotypic analysis for confirmation purposes. These were the first findings in patients in northern Brazil that we know of. |
