Comparison of Capillary Zone Electrophoresis with High-pressure Liquid Chromatography in the Evaluation of Hemoglobinopathies
| Autor: | Çakır Madenci Ö; University of Health Sciences Türkiye, Kartal Dr. Lütfi Kırdar City Hospital, Biochemistry Laboratory, İstanbul, Türkiye, Hürmeydan Ö; University of Health Sciences Türkiye, Kartal Dr. Lütfi Kırdar City Hospital, Biochemistry Laboratory, İstanbul, Türkiye, Orçun A; University of Health Sciences Türkiye, Kartal Dr. Lütfi Kırdar City Hospital, Biochemistry Laboratory, İstanbul, Türkiye, Erdoğmuş F; University of Health Sciences Türkiye, Kartal Dr. Lütfi Kırdar City Hospital, Biochemistry Laboratory, İstanbul, Türkiye |
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| Jazyk: | angličtina |
| Zdroj: | Turkish journal of haematology : official journal of Turkish Society of Haematology [Turk J Haematol] 2023 Dec 05; Vol. 40 (4), pp. 258-265. Date of Electronic Publication: 2023 Sep 19. |
| DOI: | 10.4274/tjh.galenos.2023.2023.0114 |
| Abstrakt: | Objective: The capillary zone electrophoresis (CZE) and high-performance liquid chromatography (HPLC) methods were compared in terms of HbA2 measurement for the assessment of hemoglobinopathies. Materials and Methods: CZE was compared with HPLC for the evaluation of patients without hemoglobinopathy (n=321), with β-thalassemia trait (n=113), and with common (HbD-Punjab, E, C, S/A, and S/S) and rare (HbS/D, O-Arap, Lepore, G-Coushata, Setif, Hamadan, Q-Iran, and H) variants (n=21). The reference range for HbA2 was determined by CZE. Results: Among patients without hemoglobinopathy, the median (2.5 th -97.5 th percentiles) values were 97.4% (97.0-98.0%) and 97.5% (96.6-98.4%) for HbA (p=0.060) and 2.4% (1.6-3.0%) and 2.5% (1.6-3.1%) for HbA2 (p<0.001) by HPLC and CZE, respectively. The reference range for HbA2 was 1.6-3.1% by CZE. In the comparison of methods for HbA2, there was a constant error of 0.255 (confidence interval: 0.062-0.448) and bias of 0.10% (limit of agreement: 0.33-0.53), and higher values were obtained with CZE. A strong correlation was observed between the methods (r=0.782). Interrater agreement was almost perfect for clinical diagnosis (ϰ=0.911). The two methods detected and identified the common variants similarly. All rare variants, except HbH by HPLC and HbS/D by CZE, were detected as separate peaks by both methods. Conclusion: The two methods were in agreement regarding the preliminary identification of β-thalassemia patients. Different Hb variants were detected by both methods but with possible methodological interference for HbA2 measurements. CZE is a reliable and simple alternative for the evaluation of hemoglobinopathies. The standardization of HbA2 measurements should be prioritized as more techniques become available in routine laboratory practice. Competing Interests: Conflict of Interest: No conflict of interest was declared by the authors. (©Copyright 2023 by Turkish Society of Hematology Turkish Journal of Hematology, Published by Galenos Publishing House.) |
| Databáze: | MEDLINE |
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