Endocrine disruptors in Adansonia digitata (Linn) extract induce alteration of female Wistar rats' oestrous cycle, hormone and lipid profiles.

Autor: Chibuogwu IC; Department of Theriogenology and Production, Ahmadu Bello University Zaria, Kaduna State, Nigeria; Department of Theriogenology, Faculty of Veterinary Medicine, University of Abuja, Nigeria. Electronic address: ijeoma.chibuogwu@uniabuja.edu.ng., Nwannenna AI; Department of Theriogenology and Production, Ahmadu Bello University Zaria, Kaduna State, Nigeria., Ogwu D; Department of Theriogenology and Production, Ahmadu Bello University Zaria, Kaduna State, Nigeria., Garba M; Department of Pharmaceutical Chemistry, Ahmadu Bello University Zaria, Kaduna State, Nigeria., Ubah SA; Department of Theriogenology, Faculty of Veterinary Medicine, University of Abuja, Nigeria. Electronic address: simon.ubah@uniabuja.edu.ng., Ajayi IE; Department of Veterinary Anatomy, Faculty of Veterinary Medicine, University of Abuja, Nigeria. Electronic address: e.itopa@uniabuja.edu.ng.
Jazyk: angličtina
Zdroj: General and comparative endocrinology [Gen Comp Endocrinol] 2023 Dec 01; Vol. 344, pp. 114385. Date of Electronic Publication: 2023 Sep 16.
DOI: 10.1016/j.ygcen.2023.114385
Abstrakt: Objective: Hexane-acetyl acetate (HAAF) and acetyl acetate-methanol fractions (AAMF) but not aqueous methanol (AQMF) and aqueous fractions (AQF) of Adansonia digitata Linn root bark induce reproductive effects in female Wistar rats. The current study investigated the exclusive components of HAAF, AAMF, AQMF, and AQF of Adansonia digitata Linn root bark and the effect of AAMF on the female Wistar rat's oestrous cycle progression, and hormone and lipid profiles.
Methodology: Gas chromatography and mass spectrometry explored the components of HAAF, AAMF, AQMF, and AQF. Mature female Wistar rats with a proven 4-5-days oestrous cycle were synchronised and randomly assigned into three treatment groups of 30 rats each on the day of proestrus. For seven days, rats in the different groups received 0, 150, and 300 mg kg-1 body weights of AAMF, respectively. Six rats were euthanised from each group based on a standard oestrous stage-timed sequence. The oestrous stage, hormone profile (oestrogen, progesterone, progesterone/oestrogen ratio, and FSH) and lipid profile (Total cholesterol-TC, Triglycerols, High-HD and low density-LD lipid cholesterol) of the euthanised rats were determined.
Results: tricosene, cyclopentadecanone 2-hydroxy-, oleic acid, and 9,17-octadecadienal, were exclusively found in HAAF and AAMF. The oestrous stage, serum hormone and lipids varied significantly (p < 0.05) between treatment groups. AAMF fraction induced sustained progesterone levels and depleted oestrogen levels, and TC and LDL were inversely related to serum oestrogen levels.
Discussion: The results suggest a depression of oestrogen and sustenance of progesterone-mediated effects, respectively, on GnRH surge. Oleic acid in AAMF may be responsible for its reproductive effects.
Conclusion: AAMF fraction of A. digitata (L) root bark disrupts the endocrine activity in female Wistar rats. The oleic acid component of the AAMF fraction may be responsible for modulating the activities of reproductive hormones. The authors recommend further studies to ascertain the significance of Adansonia digitata extract's oleic acid in regulating the female reproductive cycle.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: