Satureja montana and Mentha pulegium essential oils' antimicrobial properties against Pseudomonas syringae pv. actinidiae and elicitor potential through the modulation of kiwifruit hormonal defenses.

Autor: Oliveira-Fernandes J; Department of Biology, Faculty of Sciences, University of Porto, Rua Campo Alegre s/n, 4169- 007 Porto, Portugal; LAQV-REQUIMTE, Faculty of Sciences, University of Porto, Porto, Portugal., Oliveira-Pinto PR; Department of Biology, Faculty of Sciences, University of Porto, Rua Campo Alegre s/n, 4169- 007 Porto, Portugal; LAQV-REQUIMTE, Faculty of Sciences, University of Porto, Porto, Portugal. Electronic address: paulopinto@fc.up.pt., Mariz-Ponte N; Department of Biology, Faculty of Sciences, University of Porto, Rua Campo Alegre s/n, 4169- 007 Porto, Portugal; LAQV-REQUIMTE, Faculty of Sciences, University of Porto, Porto, Portugal; CIBIO-InBIO, Campus de Vairão, Universidade do Porto, Rua Padre Armando Quintas, Vairão, Portugal., Sousa RMOF; Department of Biology, Faculty of Sciences, University of Porto, Rua Campo Alegre s/n, 4169- 007 Porto, Portugal; GreenUPorto/Inov4Agro, Faculty of Sciences, University of Porto, Rua Campo Alegre, Porto, Portugal; CITAB/Inov4Agro, Universidade de Trás-os-Montes e Alto Douro, Vila Real, Portugal., Santos C; Department of Biology, Faculty of Sciences, University of Porto, Rua Campo Alegre s/n, 4169- 007 Porto, Portugal; LAQV-REQUIMTE, Faculty of Sciences, University of Porto, Porto, Portugal.
Jazyk: angličtina
Zdroj: Microbiological research [Microbiol Res] 2023 Dec; Vol. 277, pp. 127490. Date of Electronic Publication: 2023 Sep 15.
DOI: 10.1016/j.micres.2023.127490
Abstrakt: Pseudomonas syringae pv. actinidiae (Psa) is responsible for the kiwifruit bacterial canker, the most severe disease of Actinidia spp. The use in agriculture of antibiotics and cooper-based compounds is increasingly being restricted, demanding for new sustainable alternatives to current agrochemicals. We aimed to characterize the anti-Psa potential of essential oils (EOs) of Mentha pulegium and Satureja montana and investigate if they elicit the plant-host hormonal defenses. The EOs were characterized through gas-chromatography with flame ionization detector (GC-FID) and mass spectrometry (MS). Pulegone (78.6%) and carvacrol (43.5%) were the major constituents of M. pulegium and S. montana EO, respectively. Only S. montana EO showed relevant anti-Psa activity in vitro. To evaluate if the EOs also elicited host defenses, in vitro shoots were treated with 2 mg shoot -1 of EO-solution and subsequently inoculated with Psa three days later. Shoots were analyzed 10 min, three days (and 10 min after Psa-inoculation), four and ten days after EO application. The up/down regulation of RNA-transcripts for hormone biosynthesis, Psa biofilm production and virulence genes were quantified by real-time quantitative PCR (RT-qPCR). Phytohormones were quantified by High-Performance Liquid Chromatography (HPLC). S. montana EO showed the most promising results as a defense elicitor, increasing 6-benzylaminopurine (BAP) by 131.07% and reducing indole-3-acetic acid (IAA) levels by 49.19%. Decreases of salicylic acid (SA), and gibberellic acid 3 (GA3) levels by 32.55% and 33.09% respectively and an increase of abscisic acid (ABA) by 85.03%, in M. pulegium EO-treated shoots, revealed some protective post-infection effect. This is the most comprehensive research on the Psa's impact on phytohormones. It also unveils the protective influence of prior EO exposure, clarifying the plant hormonal response to subsequent infections. The results reinforce the hypothesis that carvacrol-rich S. montana EO can be a suitable disease control agent against Psa infection. Its dual action against pathogens and elicitation of host plant defenses make it a promising candidate for incorporation into environmentally friendly disease management approaches. Nonetheless, to fully leverage these promising results, further research is imperative to elucidate the EO mode of action and evaluate the long-term efficacy of this approach.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 Elsevier GmbH. All rights reserved.)
Databáze: MEDLINE
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