Effects of exercise on whole-blood transcriptome profile in children with overweight/obesity.

Autor: Altmäe S; Department of Biochemistry and Molecular Biology, Faculty of Sciences, University of Granada, Granada, Spain.; Instituto de Investigación Biosanitaria ibs.GRANADA, Granada, Spain.; Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology (CLINTEC), Karolinska Institutet and Karolinska University Hospital, Stockholm, Sweden., Plaza-Florido A; Department of Physical Education and Sports, Faculty of Sport Sciences, Sport and Health University Research Institute (iMUDS), University of Granada Granada, Granada, Spain.; Pediatric Exercise and Genomics Research Center, Department of Pediatrics, School of Medicine, University of California at Irvine, Irvine, California, USA., Esteban FJ; Systems Biology Unit, Department of Experimental Biology, Faculty of Experimental Sciences, University of Jaen, Jaen, Spain., Anguita-Ruiz A; Barcelona Institute for Global Health, ISGlobal Barcelona, Barcelona, Spain.; Department of Biochemistry and Molecular Biology II, School of Pharmacy, University of Granada Granada, Granada, Spain.; Center of Biomedical Research, Institute of Nutrition and Food Technology 'José Mataix', University of Granada, Granada, Spain.; CIBEROBN (CIBER Physiopathology of Obesity and Nutrition), Instituto de Salud Carlos III, Madrid, Spain., Krjutškov K; Competence Centre for Health Technologies, Tartu, Estonia.; Department of Obstetrics and Gynaecology, Institute of Clinical Medicine, University of Tartu, Tartu, Estonia., Katayama S; Folkhälsan Research Center, Helsinki, Finland.; Stem Cells and Metabolism Research Program, Research Programs Unit, University of Helsinki, Finland.; Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden., Einarsdottir E; Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden.; Science for Life Laboratory, Department of Gene Technology, KTH-Royal Institute of Technology, Solna, Sweden., Kere J; Folkhälsan Research Center, Helsinki, Finland.; Stem Cells and Metabolism Research Program, Research Programs Unit, University of Helsinki, Finland.; Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden., Radom-Aizik S; Pediatric Exercise and Genomics Research Center, Department of Pediatrics, School of Medicine, University of California at Irvine, Irvine, California, USA., Ortega FB; Department of Physical Education and Sports, Faculty of Sport Sciences, Sport and Health University Research Institute (iMUDS), University of Granada Granada, Granada, Spain.; Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Sweden.; Faculty of Sport and Health Sciences, University of Jyväskylä, Jyväskylä, Finland.; CIBERobn Physiopathology of Obesity and Nutrition, Granada, Spain.
Jazyk: angličtina
Zdroj: American journal of human biology : the official journal of the Human Biology Council [Am J Hum Biol] 2024 Feb; Vol. 36 (2), pp. e23983. Date of Electronic Publication: 2023 Sep 16.
DOI: 10.1002/ajhb.23983
Abstrakt: Background: The current knowledge about the molecular mechanisms underlying the health benefits of exercise is still limited, especially in childhood. We set out to investigate the effects of a 20-week exercise intervention on whole-blood transcriptome profile (RNA-seq) in children with overweight/obesity.
Methods: Twenty-four children (10.21 ± 1.33 years, 46% girls) with overweight/obesity, were randomized to either a 20-week exercise program (intervention group; n = 10), or to a no-exercise control group (n = 14). Whole-blood transcriptome profile was analyzed using RNA-seq by STRT technique with GlobinLock technology.
Results: Following the 20-week exercise intervention program, 161 genes were differentially expressed between the exercise and the control groups among boys, and 121 genes among girls (p-value <0.05), while after multiple correction, no significant difference between exercise and control groups persisted in gene expression profiles (FDR >0.05). Genes enriched in GO processes and molecular pathways showed different immune response in boys (antigen processing and presentation, infections, and T cell receptor complex) and in girls (Fc epsilon RI signaling pathway) (FDR <0.05).
Conclusion: These results suggest that 20-week exercise intervention program alters the molecular pathways involved in immune processes in children with overweight/obesity.
(© 2023 The Authors. American Journal of Human Biology published by Wiley Periodicals LLC.)
Databáze: MEDLINE
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