Recombinase-independent AAV for anterograde transsynaptic tracing.

Autor: Faress I; Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark. Islam.faress@Dandrite.au.dk.; DANDRITE, The Danish Research Institute of Translational Neuroscience, Aarhus, Denmark. Islam.faress@Dandrite.au.dk.; Center for Proteins in Memory-PROMEMO, Danish National Research Foundation, Aarhus, Denmark. Islam.faress@Dandrite.au.dk.; Department of Biomedicine, Aarhus University, Høegh-Guldbergs Gade 10, 8000, Aarhus, Denmark. Islam.faress@Dandrite.au.dk., Khalil V; Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.; DANDRITE, The Danish Research Institute of Translational Neuroscience, Aarhus, Denmark.; Center for Proteins in Memory-PROMEMO, Danish National Research Foundation, Aarhus, Denmark., Yamamoto H; DANDRITE, The Danish Research Institute of Translational Neuroscience, Aarhus, Denmark.; Department of Biomedicine, Aarhus University, Høegh-Guldbergs Gade 10, 8000, Aarhus, Denmark., Sajgo S; Department of Biosystems Science and Engineering, ETH Zurich, Basel, Switzerland., Yonehara K; DANDRITE, The Danish Research Institute of Translational Neuroscience, Aarhus, Denmark.; Department of Biomedicine, Aarhus University, Høegh-Guldbergs Gade 10, 8000, Aarhus, Denmark.; Multiscale Sensory Structure Laboratory, National Institute of Genetics, Mishima, Japan.; Department of Genetics, The Graduate University for Advanced Studies (SOKENDAI), Mishima, Japan., Nabavi S; Department of Molecular Biology and Genetics, Aarhus University, Aarhus, Denmark.; DANDRITE, The Danish Research Institute of Translational Neuroscience, Aarhus, Denmark.; Center for Proteins in Memory-PROMEMO, Danish National Research Foundation, Aarhus, Denmark.
Jazyk: angličtina
Zdroj: Molecular brain [Mol Brain] 2023 Sep 15; Vol. 16 (1), pp. 66. Date of Electronic Publication: 2023 Sep 15.
DOI: 10.1186/s13041-023-01053-7
Abstrakt: Viral transsynaptic labeling has become indispensable for investigating the functional connectivity of neural circuits in the mammalian brain. Adeno-associated virus serotype 1 (AAV1) allows for anterograde transneuronal labeling and manipulation of postsynaptic neurons. However, it is limited to delivering an AAV1 expressing a recombinase which relies on using transgenic animals or genetic access to postsynaptic neurons. We reasoned that a strong expression level could overcome this limitation. To this end, we used a self-complementary AAV of serotype 1 (scAAV1) under a strong promoter (CAG). We demonstrated the anterograde transneuronal efficiency of scAAV1 by delivering a fluorescent marker in mouse retina-superior colliculus and thalamic-amygdala pathways in a recombinase-independent manner in the mouse brain. In addition to investigating neuronal connectivity, anterograde transsynaptic AAVs with a strong promoter may be suitable for functional mapping and imaging.
(© 2023. Min Zhuo, Bong-Kiun Kaang and BioMed central Ltd.)
Databáze: MEDLINE
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