Reactive Oxygen Species-Mediated Mitophagy and Cell Apoptosis are Involved in the Toxicity of Aluminum Chloride Exposure in GC-2spd.

Autor: Peng HX; The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.; Graduate School of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China., Chai F; The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.; Graduate School of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China., Chen KH; The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.; Graduate School of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China., Huang YX; The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.; Graduate School of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China., Wei GJ; The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.; Graduate School of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China., Yuan H; The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.; Graduate School of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China., Pang YF; The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China.; Graduate School of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China., Luo SH; The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China. shihualuo99@126.com.; Graduate School of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China. shihualuo99@126.com., Wang CF; The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China. chunfang.wang@ymun.edu.cn.; Graduate School of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China. chunfang.wang@ymun.edu.cn., Chen WC; The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China. chwch3268@sina.com.; Graduate School of Youjiang Medical University for Nationalities, Baise, 533000, Guangxi, China. chwch3268@sina.com.
Jazyk: angličtina
Zdroj: Biological trace element research [Biol Trace Elem Res] 2024 Jun; Vol. 202 (6), pp. 2616-2629. Date of Electronic Publication: 2023 Sep 15.
DOI: 10.1007/s12011-023-03848-0
Abstrakt: Aluminum chloride is an inorganic polymeric coagulant commonly found in daily life and various materials. Although male reproductive toxicity has been associated with AlCl 3 exposure, the underlying mechanism remains unclear. This study aimed to examine the impact of AlCl 3 exposure on mitophagy and mitochondrial apoptosis in testicular tissue and mouse spermatocytes. Reactive oxygen species (ROS) and ATP levels were measured in GC-2spd after AlCl 3 exposure using a multifunctional enzyme labeler. The changes in mitochondrial membrane potential (MMP) and TUNEL were observed through confocal laser microscopy, and the expression of proteins associated with mitophagy and apoptosis was analyzed using Western blot. Our results demonstrated that AlCl 3 exposure disrupted mitophagy and increased apoptosis-related protein expression in testicular tissues. In the in vitro experiments, AlCl 3 exposure induced ROS production, suppressed cell viability and ATP production, and caused a decrease in MMP, leading to mitophagy and cell apoptosis in GC-2spd cells. Intervention with N-acetylcysteine (NAC) reduced ROS production and partially restored mitochondrial function, thereby reversing the resulting mitophagy and cell apoptosis. Our findings provide evidence that ROS-mediated mitophagy and cell apoptosis play a crucial role in the toxicity of AlCl 3 exposure in GC-2spd. These results contribute to the understanding of male reproductive toxicity caused by AlCl 3 exposure and offer a foundation for future research in this area.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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