Effect of cryopreservation and capacitation on expression patterns of ATP synthesis associated genes in bubaline spermatozoa.
Autor: | Sai Kiran BVS; Department of Veterinary Physiology, NTR College of Veterinary Science, Gannavaram, Sri Venkateswara Veterinary University, Andhra Pradesh, 521102, India., Srinivasa Prasad CH; Department of Veterinary Physiology, NTR College of Veterinary Science, Gannavaram, Sri Venkateswara Veterinary University, Andhra Pradesh, 521102, India. Electronic address: spchigurupati@rediffmail.com., Naik BR; Department of Veterinary Physiology, College of Veterinary Science, Tirupati, Sri Venkateswara Veterinary University, Andhra Pradesh, 517502, India., Aswani Kumar K; Department of Veterinary Biochemistry, NTR College of Veterinary Science, Gannavaram, Sri Venkateswara Veterinary University, Andhra Pradesh, 521102, India., Lavanya S; Department of Veterinary Physiology, NTR College of Veterinary Science, Gannavaram, Sri Venkateswara Veterinary University, Andhra Pradesh, 521102, India., Nikhil Kumar T; Department of Veterinary Physiology, NTR College of Veterinary Science, Gannavaram, Sri Venkateswara Veterinary University, Andhra Pradesh, 521102, India., Hyder I; Department of Veterinary Physiology and Biochemistry, College of Veterinary Science, Garividi, Sri Venkateswara Veterinary University, Andhra Pradesh, 535101, India. Electronic address: iqbvet@gmail.com. |
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Jazyk: | angličtina |
Zdroj: | Journal of thermal biology [J Therm Biol] 2023 Oct; Vol. 117, pp. 103704. Date of Electronic Publication: 2023 Sep 09. |
DOI: | 10.1016/j.jtherbio.2023.103704 |
Abstrakt: | This study investigated the expression patterns of certain ATP synthesis associated genes (GAPDHS, PGK2, ENO4 and MDH2) in fresh and frozen-thawed bubaline spermatozoa vis-à-vis capacitation. Eight adult Murrah buffalo bulls, aged 5-7 years, were randomly selected from a bull farm and fresh semen was collected once a week for four weeks (n = 32) from the selected bulls. Simultaneously, frozen straws of the same bulls were collected (n = 32, four straws were pooled per sample). The samples were divided into four groups i.e., fresh non capacitated (control/group 1), fresh capacitated (group 2), frozen non capacitated (group 3) and frozen capacitated (group 4). In all groups mRNA extraction was done. The mRNA expression of GAPDHS was significantly higher in group 3 compared to the other groups, whereas the expression of ENO4 was significantly up-regulated in group 2 and 3 compared to the group 4. Similarly, the mRNA expression of PGK2 was significantly up-regulated in group 3 whereas in case of MDH2, it was significantly higher in group 2, 3 and 4 compared group 1. It can be concluded from our study that freezing and capacitation has a significant effect on the expression dynamics of the ATP synthesis-associated genes warranting necessary interventions in handling procedures to minimise the adverse effect of freezing on ATP synthesis process to ensure persistence of fertilizing ability of sperm. Competing Interests: Declaration of competing interest The authors have declared that no competing interests exist. Grants: This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors. (Copyright © 2023. Published by Elsevier Ltd.) |
Databáze: | MEDLINE |
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